Single-Cell Sequencing Data Analysis Unveiled HDAC1 as the Therapeutic Target for Chronic Pancreatitis.

IF 2.4 4区生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Molecular Biotechnology Pub Date : 2024-01-01 Epub Date: 2023-04-06 DOI:10.1007/s12033-023-00718-x

Jie Yang, Rui Li

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引用次数: 0

Abstract

Chronic pancreatitis (CP) as a progressive inflammatory disorder, remains untreatable. The novel treatment strategy for CP is imperative. We attempted to explore the therapeutic biomarkers for CP. The single-cell sequencing data were retrieved from Gene Expression Omnibus (GEO) database. Differentially expressed genes (DEGs) in idiopathic CP were identified, followed by function and pathway annotation, and PPI network established. DEGs of interest were verified in human tissue samples. The function of candidate biomarker was determined in the murine model with CP. A total of 208 genes were specially differentially expressed in idiopathic patients. Functional enrichment analysis showed DEGs were mainly enriched in glycogen catabolic process, RNA splicing, and glucagon signaling pathway. A PPI network centered on HDAC1 was constructed. HDAC1 was overexpressed in CP patients. The murine model with CP was induced by repetitive cerulein treatment. Silencing sh-HDAC1 treatment reversed cerulein-induced inflammatory cells accumulation, high expression of TGF-β1, and collagen 1 in pancreas in vivo. HDAC1 might be served as potential biomarker for CP. The present study provided insights into the molecular mechanism of CP that may be useful in further investigations.

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单细胞测序数据分析揭示 HDAC1 是慢性胰腺炎的治疗靶点

慢性胰腺炎（CP）是一种进行性炎症性疾病，目前仍无法治疗。针对慢性胰腺炎的新型治疗策略势在必行。我们试图探索慢性胰腺炎的治疗生物标志物。我们从基因表达总库（GEO）数据库中获取了单细胞测序数据。确定了特发性心肌梗死中的差异表达基因（DEGs），随后进行了功能和通路注释，并建立了 PPI 网络。在人体组织样本中验证了感兴趣的 DEGs。在小鼠 CP 模型中确定了候选生物标志物的功能。共有 208 个基因在特发性患者中特殊差异表达。功能富集分析显示，DEGs主要富集在糖原分解过程、RNA剪接和胰高血糖素信号通路中。构建了以HDAC1为中心的PPI网络。CP患者体内HDAC1过表达。CP小鼠模型是由重复的脑磷脂治疗诱发的。沉默sh-HDAC1可逆转cerulein诱导的体内胰腺炎症细胞聚集、TGF-β1和胶原蛋白1的高表达。HDAC1 可作为潜在的 CP 生物标记物。本研究揭示了胰腺癌的分子机制，可能有助于进一步的研究。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Molecular Biotechnology 医学-生化与分子生物学

CiteScore

4.10

自引率

3.80%

发文量

165

审稿时长

6 months

期刊介绍： Molecular Biotechnology publishes original research papers on the application of molecular biology to both basic and applied research in the field of biotechnology. Particular areas of interest include the following: stability and expression of cloned gene products, cell transformation, gene cloning systems and the production of recombinant proteins, protein purification and analysis, transgenic species, developmental biology, mutation analysis, the applications of DNA fingerprinting, RNA interference, and PCR technology, microarray technology, proteomics, mass spectrometry, bioinformatics, plant molecular biology, microbial genetics, gene probes and the diagnosis of disease, pharmaceutical and health care products, therapeutic agents, vaccines, gene targeting, gene therapy, stem cell technology and tissue engineering, antisense technology, protein engineering and enzyme technology, monoclonal antibodies, glycobiology and glycomics, and agricultural biotechnology.