Enterotoxigenic profiles and submerged and interface biofilms in Bacillus cereus group isolates from foods

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2023-07-01 DOI:10.1016/j.ram.2023.01.007

Itzel-Maralhi Cruz-Facundo , Roberto Adame-Gómez , Natividad Castro-Alarcón , Jeiry Toribio-Jiménez , Yaneth Castro-Coronel , María-Cristina Santiago-Dionisio , Marco-Antonio Leyva-Vázquez , David Tafolla-Venegas , Arturo Ramírez-Peralta

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引用次数: 1

Abstract

Biofilm formation by Bacillus cereus strains is now recognized as a systematic contamination mechanism in foods; the aim of this study was to evaluate the production of submerged and interface biofilms in strains of B. cereus group in different materials, the effect of dextrose, motility, the presence of genes related to biofilms and the enterotoxigenic profile of the strains. We determine biofilm production by safranin assay, motility on semi-solid medium, toxin gene profiling and genes related to biofilm production by PCR in B. cereus group isolated from food. In this study, we observe strains used a higher production of biofilms in PVC; in the BHI broth, no submerged biofilms were found compared to phenol red broth and phenol red broth supplemented with dextrose; no strains with the ces gene were found, the enterotoxin profile was the most common the profile that includes genes for the three enterotoxins. We observed a different distribution of tasA and sipW with the origin of isolation of the strain, being more frequent in the strains isolated from eggshell. The production and type of biofilms are differential according to the type of material and culture medium used.

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食物中蜡样芽孢杆菌群分离物的产肠毒素谱和淹没和界面生物膜。

蜡样芽孢杆菌菌株的生物膜形成现在被认为是食品中的一种系统污染机制；本研究的目的是评估蜡样芽孢杆菌组菌株在不同材料中产生的浸没和界面生物膜、葡萄糖的影响、运动性、与生物膜相关的基因的存在以及菌株的肠毒素谱。我们通过藏红测定法测定了从食物中分离的蜡样芽孢杆菌组的生物膜产生、半固体培养基上的运动性、毒素基因图谱和与生物膜产生相关的基因。在这项研究中，我们观察到菌株在PVC中使用了更高产量的生物膜；在BHI肉汤中，与补充有葡萄糖的酚红肉汤和酚红肉汤相比，没有发现浸没的生物膜；没有发现携带ces基因的菌株，肠毒素图谱是最常见的，其中包括三种肠毒素的基因。我们观察到，随着菌株的分离来源，tasA和sipW的分布不同，在蛋壳分离的菌株中更为常见。生物膜的生产和类型根据所用材料和培养基的类型而不同。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.