Sasa veitchii extracts protect phenytoin-induced cell proliferation inhibition in human lip mesenchymal cells through modulation of miR-27b-5p.

IF 1.3 4区 医学 Q4 MEDICINE, RESEARCH & EXPERIMENTAL
Yosuke Tsukiboshi, Aya Ogata, Azumi Noguchi, Yurie Mikami, Satoshi Yokota, Kenichi Ogata, Hiroki Yoshioka
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引用次数: 1

Abstract

A cleft lip, with or without a cleft palate, is a common birth defect caused by environmental factors or genetic mutations. Environmental factors, such as pharmaceutical exposure in pregnant women, are known to induce cleft lip, with or without cleft palate in the child. This study aimed to investigate the protective effect of Sasa veitchii extract (SE) on phenytoin-induced inhibition of cell proliferation in human lip mesenchymal cells (KD cells) and human embryonic palatal mesenchymal cells (HEPM cells). We demonstrated that cell proliferation was inhibited by phenytoin in a dose-dependent manner in both KD and HEPM cells. Co-treatment with SE restored phenytoin-induced toxicity in KD cells but did not protect HEPM cells against phenytoin-induced toxicity. Several microRNAs (miR-27b, miR-133b, miR-205, miR-497-5p, and miR-655-3p) is reported to associate with cell proliferation in KD cells. We measured the seven kinds of microRNAs (miR27b-3p, miR-27b-5p, miR-133b, miR-205-3p, miR-205-5p, miR-497-5p, and miR-655-3p) and found that SE suppressed miR-27b-5p induced by phenytoin in KD cells. Furthermore, co-treatment with SE enhanced the expression of miR-27b-5p downstream genes (PAX9, RARA, and SUMO1). These results suggest that SE protects phenytoin-induced cell proliferation inhibition by modulating miR-27b-5p.

黄芪提取物通过调节miR-27b-5p对苯妥英诱导的人唇间充质细胞增殖抑制有保护作用。
唇裂,伴或不伴腭裂,是一种常见的先天缺陷,由环境因素或基因突变引起。众所周知,环境因素,如孕妇的药物暴露,会诱发孩子的唇裂,无论是否伴有腭裂。本研究旨在探讨黄芪提取物(SE)对苯妥英诱导的人唇间充质细胞(KD细胞)和人胚胎腭间充质细胞(HEPM细胞)增殖抑制的保护作用。我们证明,在KD和HEPM细胞中,苯妥英均以剂量依赖的方式抑制细胞增殖。与SE共处理可恢复苯妥英诱导的KD细胞毒性,但不能保护HEPM细胞免受苯妥英诱导的毒性。据报道,几种microrna (miR-27b, miR-133b, miR-205, miR-497-5p和miR-655-3p)与KD细胞的细胞增殖有关。我们检测了7种microrna (miR27b-3p、miR-27b-5p、miR-133b、miR-205-3p、miR-205-5p、miR-497-5p和miR-655-3p),发现SE抑制了苯妥英在KD细胞中诱导的miR-27b-5p。此外,与SE共处理可增强miR-27b-5p下游基因(PAX9、RARA和SUMO1)的表达。这些结果表明SE通过调节miR-27b-5p保护苯妥英诱导的细胞增殖抑制。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Biomedical Research-tokyo
Biomedical Research-tokyo 医学-医学:研究与实验
CiteScore
2.40
自引率
0.00%
发文量
19
审稿时长
>12 weeks
期刊介绍: Biomedical Research is peer-reviewed International Research Journal . It was first launched in 1990 as a biannual English Journal and later became triannual. From 2008 it is published in Jan-Apr/ May-Aug/ Sep-Dec..
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