Investigation of the inhibition of bacteria and endotoxin influx by back filtration through dialyzer membranes.

IF 1.1 4区 医学 Q4 ENGINEERING, BIOMEDICAL
Journal of Artificial Organs Pub Date : 2024-03-01 Epub Date: 2023-02-10 DOI:10.1007/s10047-023-01385-0
Tadashi Tomo, Kazuhiro Matsuyama, Junichi Michikoshi, Katsuhiro Hanada
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Abstract

We investigated the usefulness of assays using human neutrophils for radical production as well as endotoxin (ET) measurement and bacterial culture for endotoxin and bacterial influx by back filtration using dialyzers with different membrane pore diameters. Three types of dialyzers made of cellulose triacetate membrane material with different pore size FB-110EG eco, FB-110U eco, and FB-150UHβ eco were used. A circuit to generate back filtration was created. Back filtrate generated by hydraulic head pressure operation was collected. ET and bacteria were examined. Human neutrophils were exposed to back filtrate (experiments using three different membranes) and contaminated solution, and free radical production was measured using LBP-953 (Berthold) to see if there were differences in production. No bacteria were detected and the concentration of endotoxin was below the detection limit in the back filtrate from the three types of membranes and purified water. Free radical production from neutrophils in the contaminated water was highest at 4,405,750 ± 61,244 cpm (counts per minute) (mean ± SD) (P < 0.01 vs FB-150UHβ eco, FB-110U-eco, and FB-110EG eco) followed by that in back filtrate via FB-150UHβ eco, FB-110U-eco, FB-110EG eco. Radical production from neutrophils was thereby higher in the back filtrate of dialyzers with larger pore-size membranes. No bacteria were observed and the concentration of ET was below the detection limit in back filtrate from any of the membranes. However, when the reverse filtrate was exposed to neutrophils, radical production increased along with pore size, suggesting the influx of small pyrogens and other pyrogenic substances.

Abstract Image

通过透析膜反向过滤抑制细菌和内毒素流入的研究。
我们使用不同膜孔径的透析器进行反向过滤,利用人体中性粒细胞进行自由基产生和内毒素(ET)测量以及细菌培养进行内毒素和细菌流入的检测,研究了这些检测方法的实用性。使用了三种不同孔径的三醋酸纤维素膜材料制成的透析器:FB-110EG eco、FB-110U eco 和 FB-150UHβ eco。创建了一个产生反向过滤的回路。收集水头压力操作产生的反滤液。对 ET 和细菌进行检测。将人类中性粒细胞暴露于反向滤液(使用三种不同膜进行实验)和污染溶液中,使用 LBP-953 (Berthold) 测量自由基的产生情况,以确定自由基的产生是否存在差异。在三种膜的回滤液和纯净水中均未检测到细菌,内毒素浓度也低于检测限。受污染的水中嗜中性粒细胞产生的自由基最多,为 4,405,750 ± 61,244 cpm(每分钟计数)(平均值 ± SD)(P
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来源期刊
Journal of Artificial Organs
Journal of Artificial Organs 医学-工程:生物医学
CiteScore
2.80
自引率
15.40%
发文量
68
审稿时长
6-12 weeks
期刊介绍: The aim of the Journal of Artificial Organs is to introduce to colleagues worldwide a broad spectrum of important new achievements in the field of artificial organs, ranging from fundamental research to clinical applications. The scope of the Journal of Artificial Organs encompasses but is not restricted to blood purification, cardiovascular intervention, biomaterials, and artificial metabolic organs. Additionally, the journal will cover technical and industrial innovations. Membership in the Japanese Society for Artificial Organs is not a prerequisite for submission.
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