Lactiplantibacillus plantarum BW2013 protects mucosal integrity and modulates gut microbiota of mice with colitis.

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2023-04-01 DOI:10.1139/cjm-2022-0092

Xiaohui Niu, Qian Li, Na Luan, Jia Liu, Michael Zhang, Jun An, Zuming Li, Zhihui Bai, Ran Xia, Zhichao Wu

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引用次数: 2

Abstract

This study explored the effects of Lactiplantibacillus plantarum (previously Lactobacillus plantarum) BW2013 on mucosal integrity and gut microbiota of mice with dextran sulfate sodium (DSS)-induced colitis. The results show that the clinical symptoms in DSS-modelled ulcerative colitis (UC) were improved by L. plantarum BW2013 via decreasing disease activity index scores and suppressing inflammatory cell infiltration. Furthermore, L. plantarum BW2013 decreased the levels of diamine oxidase activity, myeloperoxidase, and D-lactic acid. The mRNA expression of ZO-1, occludin, and claudin-1 was upregulated by L. plantarum BW2013, which also increased IL-10 and reduced TNF-α, IL-1β, and IL-6 in the colon. 16S rDNA sequencing showed that L. plantarum BW2013 enhanced α-diversity. L. plantarum BW2013 upregulated significantly the abundance of unidentfied Lachnospiraceae, Lactococcus, Rikenella, Lactobacillus, and Odoribacter, which had an inhibitory effect on inflammation and a protective effect on the integrity of the mucosa. These results demonstrate that L. plantarum BW2013 alleviates DSS-modelled UC by protecting mucosal integrity and ameliorating the composition of gut microbiota.

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植物乳杆菌BW2013保护结肠炎小鼠的粘膜完整性并调节肠道微生物群。

本研究探讨植物乳杆菌BW2013对葡聚糖硫酸钠(DSS)诱导结肠炎小鼠黏膜完整性和肠道菌群的影响。结果表明，植物乳杆菌BW2013通过降低疾病活动指数评分和抑制炎症细胞浸润来改善dss模型溃疡性结肠炎(UC)的临床症状。此外，L. plantarum BW2013降低了二胺氧化酶、髓过氧化物酶和d -乳酸的活性。植物L. plantarum BW2013上调ZO-1、occludin、claudin-1 mRNA表达，上调结肠组织中IL-10，降低TNF-α、IL-1β、IL-6。16S rDNA测序结果表明，L. plantarum BW2013增强了α-多样性。L. plantarum BW2013显著上调未鉴定的毛螺杆菌科、乳球菌、里氏菌、乳杆菌和臭杆菌的丰度，对炎症有抑制作用，对粘膜完整性有保护作用。这些结果表明，L. plantarum BW2013通过保护粘膜完整性和改善肠道菌群组成来缓解dss模型UC。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.