A surprising finding: The curious case of a tongue lesion misdiagnosed as paracoccidioidomycosis

IF 1.5 4区 生物学 Q4 MYCOLOGY
Bert Gerrits van den Ende , Anderson Messias Rodrigues , Rosane Christine Hahn , Ferry Hagen
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引用次数: 0

Abstract

Background

Paracoccidioidomycosis is an endemic mycosis caused by members of the Paracoccidioides genus. Brazil remains the focus area and, to a lesser extent, the disease has been reported from Argentina, Colombia and Venezuela.

Aims

A Venezuelan Paracoccidioides brasiliensis strain, isolated from a patient diagnosed with chronic multifocal paracoccidioidomycosis, was subjected to whole genome sequencing to provide more insight about Paracoccidioides outside the endemic focus area.

Methods

P. brasiliensis strain CBS 118890 was whole genome sequenced using nanopore; library preparation with the ‘native barcoding genomic DNA kit’ was followed by sequencing on Flongle and MinION flowcells. Batches of strain CBS 118890 were re-identified by sequencing the internal transcribed spacer (ITS) region, and final identification was made based on phylogenetic analysis.

Results

Surprisingly, the Venezuelan P. brasiliensis strain CBS 118890 turned out to be a Nannizziopsis species. The batches of this strain were ITS sequenced followed by phylogenetic analysis and resulted in the final identification of Nannizziopsis arthrosporioides.

Conclusions

Nannizziopsis infections are commonly seen in a wide variety of reptiles, but are particularly rare in human infections. This case underlines the need for molecular characterization of cases that clinically mimic paracoccidioidomycosis but that are serologically negative for Paracoccidioides.

一个令人惊讶的发现:舌头病变误诊为副球孢子菌病的奇怪病例
背景副球虫病是由副球虫属成员引起的地方性真菌病。巴西仍然是重点地区,阿根廷、哥伦比亚和委内瑞拉也在较小程度上报告了这种疾病。AimsA从一名被诊断为慢性多灶性巴西副球虫病的患者身上分离出的委内瑞拉副球虫菌株进行了全基因组测序,以提供更多关于地方病重点地区以外副球虫的信息。方法P。利用纳米孔对巴西菌株CBS 118890进行全基因组测序;用“天然条形码基因组DNA试剂盒”制备文库后,对Flongle和MinION流细胞进行测序。通过对内部转录间隔区(ITS)进行测序,对CBS 118890菌株进行了重新鉴定,并基于系统发育分析进行了最终鉴定。结果委内瑞拉巴西P.brasiliensis菌株CBS 118890为Nannizziopsis种。对该菌株进行ITS测序,然后进行系统发育分析,最终鉴定为节孢子虫Nannizziopsis。结论Nannizziopsis感染常见于多种爬行动物,但在人类感染中尤为罕见。该病例强调了对临床上模拟副球虫病但血清学上副球虫阴性的病例进行分子表征的必要性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
CiteScore
1.90
自引率
0.00%
发文量
17
审稿时长
81 days
期刊介绍: Revista Iberoamericana de Micología (Ibero-American Journal of Mycology) is the official journal of the Asociación Española de Micología, Asociación Venezolana de Micología and Asociación Argentina de Micología (The Spanish, Venezuelan, and Argentinian Mycology Associations). The Journal gives priority to publishing articles on studies associated with fungi and their pathogenic action on humans and animals, as well as any scientific studies on any aspect of mycology. The Journal also publishes, in Spanish and in English, original articles, reviews, mycology forums, editorials, special articles, notes, and letters to the editor, that have previously gone through a scientific peer review process.
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