Metabolomic Alterations in Mammary Glands from Pubertal Mice Fed a High-Fat Diet.

IF 2.3 Q3 NUTRITION & DIETETICS
Lin Yan, Bret M Rust, Sneha Sundaram, Michael R Bukowski
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Abstract

Dietary malpractice is a risk factor for obesity. This study tested the hypothesis that consumption of a high-fat diet alters mammary metabolome in pubertal mice. We performed untargeted metabolomic analysis of primary metabolism on mammary glands from pubertal mice fed the AIN93G standard diet or a high-fat diet (HFD) for 3 weeks. We identified 97 metabolites for statistical comparisons. The HFD altered the amino acid metabolism considerably. This included elevated expression of branched-chain amino acids, non-essential amino acids (aspartic acid and glutamic acid), and methionine sulfoxide (oxidized methionine) and an alteration in the aminoacyl-tRNA biosynthesis pathway. Furthermore, elevations of fumaric acid and malic acid (both are citrate cycle intermediates) and glyceric acid (its phosphate derivatives are intermediates of glycolysis) in HFD-fed mice suggest an acceleration of both citrate cycle and glycolysis. Lower expression of glycerol, oleic acid, and palmitoleic acid, as well as decreased mammary expression of genes encoding lipid metabolism (Acaca, Fads1, Fasn, Scd1, and Srebf1) in HFD-fed mice indicate an attenuated lipid metabolism in the presence of adequate dietary fat. In conclusion, consumption of the HFD for 3 weeks alters metabolic profile of pubertal mammary glands. This alteration may affect mammary development and growth in pubertal mice.

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高脂肪饮食对青春期小鼠乳腺代谢组学的影响。
饮食不当是肥胖的一个危险因素。这项研究验证了高脂肪饮食会改变青春期小鼠乳腺代谢组的假设。我们对饲喂AIN93G标准饮食或高脂饮食(HFD) 3周的发育期小鼠乳腺的初级代谢进行了非靶向代谢组学分析。我们鉴定出97种代谢物进行统计比较。HFD显著改变了氨基酸代谢。这包括支链氨基酸、非必需氨基酸(天冬氨酸和谷氨酸)和蛋氨酸亚砜(氧化蛋氨酸)的表达升高,以及氨基酰基- trna生物合成途径的改变。此外,富马酸和苹果酸(两者都是柠檬酸循环的中间产物)和甘油三酸(其磷酸盐衍生物是糖酵解的中间产物)在饲喂hfd的小鼠体内的升高表明柠檬酸循环和糖酵解都加速了。低表达的甘油,油酸和棕榈油酸,以及乳腺编码脂质代谢基因(Acaca, Fads1, Fasn, Scd1和Srebf1)在hfd喂养的小鼠中表达减少,表明存在足够的膳食脂肪的脂质代谢减弱。总之,食用HFD 3周改变了青春期乳腺的代谢谱。这种改变可能影响发育期小鼠的乳腺发育和生长。
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来源期刊
Nutrition and Metabolic Insights
Nutrition and Metabolic Insights NUTRITION & DIETETICS-
CiteScore
3.30
自引率
0.00%
发文量
27
审稿时长
8 weeks
期刊介绍: Nutrition and Metabolic Insights is a peer-reviewed, open-access online journal focusing on all aspects of nutrition and metabolism. This encompasses nutrition, including the biochemistry of metabolism, exercise and associated physical processes and also includes clinical articles that relate to metabolism, such as obesity, lipidemias and diabetes. It includes research at the molecular, cellular and organismal levels. This journal welcomes new manuscripts for peer review on the following topics: Nutrition, including the biochemistry of metabolism, Exercise and associated physical processes, Clinical articles that relate to metabolism, such as obesity, lipidemias and diabetes, Research at the molecular, cellular and organismal levels, Other areas of interest include gene-nutrient interactions, the effects of hormones, models of metabolic function, macronutrient interactions, outcomes of changes in diet, and pathophysiology.
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