Saposhnikovia divaricata root and its major components ameliorate inflammation and altered gut microbial diversity and compositions in DSS-induced colitis

IF 2.8 4区 医学 Q2 INTEGRATIVE & COMPLEMENTARY MEDICINE
Saruul Erdenebileg , Yang-Ju Son , Myungsuk Kim , Sarangerel Oidovsambuu , Kwang Hyun Cha , Jaeyoung Kwon , Da Seul Jung , Chu Won Nho
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引用次数: 0

Abstract

Background

The root of Saposhnikovia divaricata (Turcz.) Schischk is a well-known traditional medicinal plant, containing various bioactive compounds with anti-inflammatory, antioxidant, and analgesic properties. However, no scientific studies have validated its clinical use as an anti-inflammatory agent against inflammatory bowel disease (IBD). This study aimed to investigate whether the root extract of S. divaricata ameliorates IBD and induces gut microbial alteration, using a RAW 264.7 cell line and a DSS-induced colitis mouse model.

Methods

To investigate the anti-inflammatory effects and alleviation of IBD, using a methanol extract of Saposhnikovia divaricata (Turcz.) Schischk. root (MESD), RAW 264.7, murine macrophages and a dextran sodium sulfate (DSS)-induced colitis mouse model were employed. 16S rRNA gene sequencing was conducted to determine the alterations in the gut microbiota of mice with DSS-induced colitis.

Results

MESD significantly decreased nitric oxide (NO) and inflammatory cytokine levels in lipopolysaccharide (LPS)-induced RAW 264.7 cells in vitro. Oral administration of MESD reduced the expression of inflammatory cytokines in the colons of mice with DSS-induced colitis. Additionally, MESD inhibited the abundance of Clostridium sensu stricto 1 and enhanced the predicted functional pathways, including l-glutamate degradation VIII (to propanoic acid). Seven compounds with anti-inflammatory properties were isolated from the MESD. Among them, 3′-O-acetylhamaudol and 3′-O-angeloylhamaudol exhibited strong anti-inflammatory effects in vitro.

Conclusion

Overall, MESD may be a potential natural product for the treatment of IBD by lowering inflammatory cytokine levels and altering gut microbiota composition.

Abstract Image

菝葜根及其主要成分可改善dss诱导的结肠炎的炎症和改变肠道微生物的多样性和组成
背景:土属植物Saposhnikovia divaricata石竹是一种著名的传统药用植物,含有多种生物活性化合物,具有抗炎、抗氧化、镇痛等作用。然而,没有科学研究证实其作为抗炎症性肠病(IBD)的抗炎剂的临床应用。本研究旨在通过RAW 264.7细胞系和dss诱导的结肠炎小鼠模型,研究鸢尾根提取物是否能改善IBD并诱导肠道微生物改变。方法采用甲醇提取物对IBD的抗炎和缓解作用进行研究。Schischk。采用小鼠巨噬细胞和右旋糖酐硫酸钠(DSS)诱导的小鼠结肠炎模型。通过16S rRNA基因测序来确定dss诱导结肠炎小鼠肠道菌群的变化。结果smesd显著降低脂多糖(LPS)诱导的RAW 264.7细胞的一氧化氮(NO)和炎性细胞因子水平。口服MESD可降低dss诱导结肠炎小鼠结肠中炎症细胞因子的表达。此外,MESD抑制了严格感梭菌1的丰度,并增强了预测的功能途径,包括l-谷氨酸降解VIII(到丙酸)。从MESD中分离到7个具有抗炎作用的化合物。其中,3′- o -乙酰哈马多尔和3′- o - angeloyl哈马多尔在体外表现出较强的抗炎作用。综上所述,MESD可能是通过降低炎症细胞因子水平和改变肠道菌群组成来治疗IBD的潜在天然产物。
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来源期刊
Integrative Medicine Research
Integrative Medicine Research Medicine-Complementary and Alternative Medicine
CiteScore
6.50
自引率
2.90%
发文量
65
审稿时长
12 weeks
期刊介绍: Integrative Medicine Research (IMR) is a quarterly, peer-reviewed journal focused on scientific research for integrative medicine including traditional medicine (emphasis on acupuncture and herbal medicine), complementary and alternative medicine, and systems medicine. The journal includes papers on basic research, clinical research, methodology, theory, computational analysis and modelling, topical reviews, medical history, education and policy based on physiology, pathology, diagnosis and the systems approach in the field of integrative medicine.
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