Nitrite improved nitrification efficiency and enriched ammonia-oxidizing archaea and bacteria in the simultaneous nitrification and denitrification process

IF 7.2 2区环境科学与生态学 Q1 ENGINEERING, ENVIRONMENTAL

Water Research X Pub Date : 2023-10-02 DOI:10.1016/j.wroa.2023.100204

Yu Xiang , Tengzhi Zhou , Siping Deng , Zhiyu Shao , Yiwen Liu , Qiang He , Hongxiang Chai

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Abstract

Simultaneous nitrification and denitrification (SND) is effective and energy-saving for wastewater treatment. As an inevitable intermediate product in the SND process, nitrite affects the efficiency of ammonia oxidation and the composition of nitrifiers. To investigate the impact of nitrite on ammonia oxidation efficiency, two reactors performing SND were respectively operated without nitrite (R1 as control) and with 20 mg N/L nitrite addition (R2 as experimental). The total nitrogen removal efficiency was 74.5% in R1 while 99.0% in R2. With nitrite addition (i.e., 20 mg N/L), the ammonia removal rate in R2 increased to 4.5 times of that in R1. The ammonia-oxidizing archaea (AOA) and ammonia-oxidizing bacteria (AOB) contributed to respective around 46.9% and 41.8% ammonia removal in R2 based on the results of experiments with specific inhibitors. The number of respective AOA and AOB ammonia monooxygenase gene (amoA) copies increased by 280 and 30 times due to nitrite addition, according to the qPCR results. The high-throughput sequencing results illustrated the increase of dominant AOB species from 0.40% in R1 to 1.59% in R2 and the phylogenetic tree analysis revealed a close link to Nitrosospira multiformis. These results indicated that the ammonia removal efficiency was improved and AOA/AOB were enriched by nitrite addition. The specific nitrite reductases in AOA and AOB boosted the adaptation of nitrite addition. This study demonstrated the positive impacts of nitrite addition on the ammonia removal efficiency and rate in the SND process.

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亚硝酸盐在同步硝化反硝化过程中提高了硝化效率，富集了氨氧化古菌和细菌

同时硝化反硝化(SND)是一种高效节能的污水处理方法。亚硝酸盐作为SND过程中不可避免的中间产物，影响氨氧化效率和硝化器的组成。为研究亚硝酸盐对氨氧化效率的影响，分别在不添加亚硝酸盐的情况下(R1为对照)和添加20 mg N/L亚硝酸盐的情况下(R2为实验)运行两个SND反应器。总氮去除率R1为74.5%，R2为99.0%。添加亚硝酸盐(即20 mg N/L)后，R2的氨去除率提高到R1的4.5倍。根据特定抑制剂的实验结果，氨氧化古菌(AOA)和氨氧化细菌(AOB)在R2中的氨去除率分别约为46.9%和41.8%。qPCR结果显示，添加亚硝酸盐后，AOA和AOB氨单加氧酶基因(amoA)的拷贝数分别增加了280倍和30倍。高通量测序结果显示，优势种AOB从R1的0.40%增加到R2的1.59%，系统进化树分析显示与多形亚硝化螺旋体有密切联系。结果表明，亚硝酸盐的加入提高了氨氮的去除率，并富集了AOA/AOB。AOA和AOB中特异的亚硝酸盐还原酶增强了亚硝酸盐添加的适应性。研究表明，在SND工艺中，亚硝酸盐的加入对氨氮的去除效率和速率有积极的影响。

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来源期刊

Water Research X Environmental Science-Water Science and Technology

CiteScore

12.30

自引率

1.30%

发文量

期刊介绍： Water Research X is a sister journal of Water Research, which follows a Gold Open Access model. It focuses on publishing concise, letter-style research papers, visionary perspectives and editorials, as well as mini-reviews on emerging topics. The Journal invites contributions from researchers worldwide on various aspects of the science and technology related to the human impact on the water cycle, water quality, and its global management.