Evaluation of the validity of the pre-marriage mean corpuscular volume value as a predictive test for b-thalassemia carrier status.

IF 2 4区 医学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY
Ali A Almomani, Ala'a S Shraim, Ali M Atoom, Majeed Bayan A Abdel, Jehad F Alhmoud
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引用次数: 0

Abstract

Background: The national mandatory premarital screening test is based on mean corpuscular volume (MCV) > 80 fL value for the detection of β-thalassemia to provide acceptance for marriage. The objective of this study is to assess the efficacy of MCV as a screening test for β-thalassemia trait in the present population.

Methods: This study was conducted on 418 blood samples collected from adult individuals. The diagnosis of β-thalassemia carrier was given to those having HbA2 values equal to or above 3.5%. The diagnostic reliability of different RBC indices and formulas in discriminating cases of β-thalassemia trait were evaluated. Finally, a new index called "Momani" was determined based on MCV, RDW and RBC count.

Results: β-thalassemia trait was identified in 10% of the cases. The measured MCV value was significantly lower in β-thalassemia carrier group compared to non-carrier group (p = <0.001). MCV value and RBC count showed a higher diagnostic reliability than other RBC indices. We found that MCV ≤ 74.45 fL is more suitable cutoff value of MCV with 86.2% specificity, 71.4% sensitivity, 36.6% positive predictive value, and 96.4% negative predictive value. Finally, our index "Momani" was found to be useful in predicting carrier and paralleled the performance of Sirdah, Mentzer, and Ehsani indices.

Conclusions: MCV<80 is a useful but not a perfect cutoff point for the screening of β-thalassemia carriers from noncarriers. The diagnostic accuracy of MCV can be improved by selecting a new cutoff value. Moreover, "Momani" index shows good discrimination ability in diagnosing β-thalassemia carrier in our population.

评估婚前平均血球容积值作为 b 型地中海贫血症携带者状态预测测试的有效性。
背景:国家强制婚前筛查测试以平均血球容积(MCV)> 80 fL 值为基础,用于检测 β 地中海贫血症,以便为婚姻提供依据。本研究的目的是评估 MCV 作为β-地中海贫血特质筛查测试在当前人群中的有效性:本研究采集了 418 份成人血样。HbA2值等于或高于3.5%者被诊断为β地中海贫血携带者。评估了不同的红细胞指数和公式在鉴别 β-地中海贫血特质病例方面的诊断可靠性。最后,根据 MCV、RDW 和 RBC 计数确定了一种名为 "Momani "的新指数。与非携带者组相比,β-地中海贫血携带者组的 MCV 测量值明显偏低(P = 结论:β-地中海贫血携带者组的 MCV 测量值明显低于非携带者组):MCV
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来源期刊
Journal of Medical Biochemistry
Journal of Medical Biochemistry BIOCHEMISTRY & MOLECULAR BIOLOGY-
CiteScore
3.00
自引率
12.00%
发文量
60
审稿时长
>12 weeks
期刊介绍: The JOURNAL OF MEDICAL BIOCHEMISTRY (J MED BIOCHEM) is the official journal of the Society of Medical Biochemists of Serbia with international peer-review. Papers are independently reviewed by at least two reviewers selected by the Editors as Blind Peer Reviews. The Journal of Medical Biochemistry is published quarterly. The Journal publishes original scientific and specialized articles on all aspects of clinical and medical biochemistry, molecular medicine, clinical hematology and coagulation, clinical immunology and autoimmunity, clinical microbiology, virology, clinical genomics and molecular biology, genetic epidemiology, drug measurement, evaluation of diagnostic markers, new reagents and laboratory equipment, reference materials and methods, reference values, laboratory organization, automation, quality control, clinical metrology, all related scientific disciplines where chemistry, biochemistry, molecular biology and immunochemistry deal with the study of normal and pathologic processes in human beings.
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