LINC00467 induces melanoma deterioration by targeting miR-485-5p/p21 activated kinase 1.

IF 2 4区医学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Journal of Medical Biochemistry Pub Date : 2023-03-15 DOI:10.5937/jomb0-39708

Zhoujing Ji, Jie Zhang, Lili Zhang, Shengju Yang, Yangcheng Li, Lixiong Gu

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Abstract

Background: The purpose of the current research was to investigate the biological roles of LINC00467 in inducing melanoma deterioration.

Methods: Differential level of LINC00467 in melanoma tissues and its prognostic value were analyzed in GEPIA, which were further confirmed in clinical samples we collected. Regulatory effects of LINC00467 on proliferation, migration and invasion capacities of A375 and SKMEL1 cell lines were examined by a series of functional experiments. Potential downstream targets of LINC00467 were identified through dual-luciferase reporter assay, and their synergistic role in melanoma process was finally explored by rescue experiments.

Results: LINC00467 was up-regulated in melanoma samples, but it did not have a prognostic potential in melanoma. LINC00467 has the capacities to stimulate proliferation, migration and invasion of A375 and SKMEL1 cell lines. The feedback loop LINC00467/miR-485-5p/PAK1 was identified, which was responsible for inducing melanoma deterioration.

Conclusions: LINC00467 stimulates proliferation, migration and invasion capacities of melanoma via targeting miR-485-5p to upregulate PAK1, which provides potential targets for treatment of melanoma.

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LINC00467通过靶向miR-485-5p/p21活化激酶1诱导黑色素瘤恶化。

背景:本研究旨在探讨LINC00467在诱导黑色素瘤恶化中的生物学作用。方法:分析GEPIA患者黑色素瘤组织中LINC00467的差异水平及其预后价值，并在收集的临床样本中进一步证实。通过一系列功能实验检测LINC00467对A375和SKMEL1细胞系增殖、迁移和侵袭能力的调控作用。通过双荧光素酶报告基因实验确定LINC00467的潜在下游靶点，最后通过抢救实验探讨其在黑色素瘤过程中的协同作用。结果:LINC00467在黑色素瘤样本中表达上调，但在黑色素瘤中不具有预后潜力。LINC00467具有刺激A375和SKMEL1细胞系增殖、迁移和侵袭的能力。发现了诱导黑色素瘤恶化的反馈回路LINC00467/miR-485-5p/PAK1。结论:LINC00467通过靶向miR-485-5p上调PAK1，刺激黑色素瘤的增殖、迁移和侵袭能力，为治疗黑色素瘤提供了潜在靶点。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Medical Biochemistry BIOCHEMISTRY & MOLECULAR BIOLOGY-

CiteScore

3.00

自引率

12.00%

发文量

审稿时长

>12 weeks

期刊介绍： The JOURNAL OF MEDICAL BIOCHEMISTRY (J MED BIOCHEM) is the official journal of the Society of Medical Biochemists of Serbia with international peer-review. Papers are independently reviewed by at least two reviewers selected by the Editors as Blind Peer Reviews. The Journal of Medical Biochemistry is published quarterly. The Journal publishes original scientific and specialized articles on all aspects of clinical and medical biochemistry, molecular medicine, clinical hematology and coagulation, clinical immunology and autoimmunity, clinical microbiology, virology, clinical genomics and molecular biology, genetic epidemiology, drug measurement, evaluation of diagnostic markers, new reagents and laboratory equipment, reference materials and methods, reference values, laboratory organization, automation, quality control, clinical metrology, all related scientific disciplines where chemistry, biochemistry, molecular biology and immunochemistry deal with the study of normal and pathologic processes in human beings.