Heterologous phasin expression in Rhodopseudomonas palustris CGA009 for bioplastic production from lignocellulosic biomass

IF 3.7 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Brandi Brown , Cheryl Immethun , Adil Alsiyabi , Dianna Long , Mark Wilkins , Rajib Saha
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引用次数: 11

Abstract

Rhodopseudomonas palustris CGA009 is a metabolically robust microbe that can utilize lignin breakdown products to produce polyhydroxyalkanoates (PHAs), biopolymers with the potential to replace conventional plastics. Our recent efforts suggest PHA granule formation is a limiting factor for maximum production of the bioplastic poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) by R. palustris. The Phap1 phasin (phaP1) from the PHB-producing model bacterium Cupriavidus necator H16 was expressed in R. palustris with the aim of overproducing PHBV from the lignin breakdown product p-coumarate by fostering smaller and more abundant granules. Expression of phaP1 yielded PHBV production from R. palustris aerobically (0.7 g/L), which does not occur in the wild-type strain, and led to a significantly higher PHBV titer than wild-type anaerobic production (0.41 g/L). The 3HV fractions were also significantly increased under both anaerobic and aerobic conditions, which boosts thermomechanical properties and potential for application. Thus, heterologous phasin expression in R. palustris provides flexibility for industrial processing and could foster compositional changes in copolymers with better thermomechanical properties compared to PHB alone.

异源phasin在palustris红假单胞菌CGA009中的表达,用于木质纤维素生物质的生物塑料生产
palustris红假单胞菌CGA009是一种代谢强大的微生物,可以利用木质素分解产物生产聚羟基烷酸酯(PHAs),这是一种具有替代传统塑料潜力的生物聚合物。我们最近的研究表明,PHA颗粒的形成是R. palustris最大限度地生产生物塑料聚(3-羟基丁酸酯-co-3-羟基戊酸酯)(PHBV)的限制因素。产phb模式细菌Cupriavidus necator H16中的Phap1 phasin (Phap1)在palustris中表达,目的是通过培养更小、更丰富的颗粒,从木质素分解产物p-coumarate中过量生产PHBV。phaP1的表达使palustris菌株产生PHBV (0.7 g/L),这在野生型菌株中没有发生,并且导致PHBV滴度显著高于野生型菌株(0.41 g/L)。在厌氧和好氧条件下,3HV组分也显著增加,这提高了热机械性能和应用潜力。因此,与单独PHB相比,异源phasin在R. palustris中的表达为工业加工提供了灵活性,并且可以促进共聚物的组成变化,从而具有更好的热机械性能。
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来源期刊
Metabolic Engineering Communications
Metabolic Engineering Communications Medicine-Endocrinology, Diabetes and Metabolism
CiteScore
13.30
自引率
1.90%
发文量
22
审稿时长
18 weeks
期刊介绍: Metabolic Engineering Communications, a companion title to Metabolic Engineering (MBE), is devoted to publishing original research in the areas of metabolic engineering, synthetic biology, computational biology and systems biology for problems related to metabolism and the engineering of metabolism for the production of fuels, chemicals, and pharmaceuticals. The journal will carry articles on the design, construction, and analysis of biological systems ranging from pathway components to biological complexes and genomes (including genomic, analytical and bioinformatics methods) in suitable host cells to allow them to produce novel compounds of industrial and medical interest. Demonstrations of regulatory designs and synthetic circuits that alter the performance of biochemical pathways and cellular processes will also be presented. Metabolic Engineering Communications complements MBE by publishing articles that are either shorter than those published in the full journal, or which describe key elements of larger metabolic engineering efforts.
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