Growth hormone enhances the CD34+ stem cells repopulation of the male albino rat thymus gland in cyclophosphamide induced injury: immunohistochemical and electron microscopic study.

IF 1.1 4区医学 Q4 MICROSCOPY

Ultrastructural Pathology Pub Date : 2023-01-29 DOI:10.1080/01913123.2023.2170510

Amira I Shrief, Walaa H E Hamed, Shireen A Mazroa, Amal M Moustafa

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引用次数: 0

Abstract

Cyclophosphamide (CP) is a chemotherapeutic drug that has a harmful effect on the immune system. Growth hormone (GH) is a peptide hormone that can enhance thymic functions in cases of immunosuppression. Therefore, the present study was performed to study the possible protective effect of growth hormone on cyclophosphamide-induced changes in the rat thymus gland. Sixty-four adult male albino rats were used and divided into three main groups. Group I (Control group). Group II (CP group) received 200 mg/kg body weight CP by a single intra-peritoneal injection. Group III (CP& GH group) received GH in a dose of 2 mg/kg body weight/day by subcutaneous injection starting 5 days before cyclophosphamide injection till the end of the experiment. Administration of CP (Group II) resulted in marked histopathological changes in thymus. Thymic cortex showed depletion of thymoblasts. There was a decrease in CD34 immune positively stained stem cells and an increase in CD68 immune positively stained macrophages. Ultrastructurally, thymoblasts were markedly degenerated and the most of epithelial reticular cells were vacuolated. Administration of GH (group III) showed preservation of the histological structure of the thymus. In conclusion, growth hormone could protect against cyclophosphamide induced thymic damage.

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生长激素增强环磷酰胺诱导损伤的雄性白化大鼠胸腺的 CD34+ 干细胞再增殖：免疫组化和电子显微镜研究。

环磷酰胺（CP）是一种对免疫系统有害的化疗药物。生长激素（GH）是一种肽类激素，可在免疫抑制情况下增强胸腺功能。因此，本研究旨在探讨生长激素对环磷酰胺诱导的大鼠胸腺变化可能产生的保护作用。研究使用了 64 只成年雄性白化大鼠，并将其分为三大组。第一组（对照组）第二组（CP 组）一次性腹腔注射 200 毫克/千克体重的 CP。第三组（CP& GH 组）在注射环磷酰胺前 5 天开始皮下注射 GH，剂量为 2 毫克/千克体重/天，直至实验结束。CP组（II组）的胸腺组织病理变化明显。胸腺皮质显示胸腺母细胞减少。CD34 免疫阳性染色干细胞减少，CD68 免疫阳性染色巨噬细胞增加。在超微结构上，胸腺母细胞明显退化，大部分上皮网状细胞呈空泡状。给予生长激素（第 III 组）后，胸腺的组织学结构得以保留。总之，生长激素可以防止环磷酰胺引起的胸腺损伤。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Ultrastructural Pathology 医学-病理学

CiteScore

2.00

自引率

10.00%

发文量

审稿时长

6-12 weeks

期刊介绍： Ultrastructural Pathology is the official journal of the Society for Ultrastructural Pathology. Published bimonthly, we are the only journal to be devoted entirely to diagnostic ultrastructural pathology. Ultrastructural Pathology is the ideal journal to publish high-quality research on the following topics: Advances in the uses of electron microscopic and immunohistochemical techniques Correlations of ultrastructural data with light microscopy, histochemistry, immunohistochemistry, biochemistry, cell and tissue culturing, and electron probe analysis Important new, investigative, clinical, and diagnostic EM methods.