Diversity of hexavalent chromium-reducing bacteria and physicochemical properties of the Kanpur tannery wastewater

IF 9 Q1 ENVIRONMENTAL SCIENCES

Environmental Chemistry and Ecotoxicology Pub Date : 2023-01-01 DOI:10.1016/j.enceco.2023.11.001

Ashish Kumar , Rupali Mishra , Alok Srivastava , Sanjay Kumar Garg , Vijay Pal Singh , Pankaj Kumar Arora

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Abstract

The tannery effluent sample was collected from a tannery located in Jajmau area of Kanpur, India and subjected to physicochemical analysis. The tannery effluent was brownish in color with a foul odor. It was alkaline in nature and characterized by high biological oxygen demand (BOD), chemical oxygen demand (COD), total solids (TS), total dissolved solids (TDS) and high amounts of chromium. Total eighteen chromium resistant bacteria were isolated from the tannery effluent sample. Based on the 16S rRNA gene sequencing and phylogenetic analysis, these eighteen bacteria belong to seven different genera including Bacillus, Pseudomonas, Kocuria, Cellulosimicrbium, Klebsiella, Microbacterium, Brucella. The members of genus Bacillus constitute 28% of total chromium transforming isolates; followed by members of genus Microbacterium (17%) Brucella (17%), Pseudomonas (11%), Cellulosimicrobium (11%), Kocuria (11%) and Klebsiella (5%). Furthermore, all eighteen bacteria were screened for their ability to reduce hexavalent chromium with increasing concentrations (600–1200 ppm) of potassium dichromate. Results showed that one bacterium, Bacillus licheniformis strain KNP exhibited hexavalent chromium reduction up to concentrations of 1000 ppm. This bacterium was selected for further study.

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坎普尔制革厂废水中六价铬还原菌的多样性及其理化性质

制革厂废水样本是从位于印度坎普尔Jajmau地区的一家制革厂收集的，并进行了物理化学分析。制革厂的废水呈褐色，有一股恶臭。它是碱性的，具有高生物需氧量(BOD)、化学需氧量(COD)、总固形物(TS)、总溶解固形物(TDS)和高铬含量的特点。从制革废水中共分离出18株耐铬细菌。根据16S rRNA基因测序和系统发育分析，这18种细菌分别属于芽孢杆菌、假单胞菌、Kocuria、cellulosimicrum、克雷伯氏菌、微细菌、布鲁氏菌等7个属。芽孢杆菌属成员占总铬转化菌株的28%;其次是微杆菌属(17%)、布鲁氏菌属(17%)、假单胞菌属(11%)、微生物纤维素菌属(11%)、科库利菌属(11%)和克雷伯菌属(5%)。此外，对所有18种细菌进行了筛选，以确定它们在增加重铬酸钾浓度(600-1200 ppm)时还原六价铬的能力。结果表明，一种细菌，地衣芽孢杆菌(Bacillus licheniformis)菌株KNP在1000ppm浓度下可以还原六价铬。这种细菌被选作进一步的研究。

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来源期刊

Environmental Chemistry and Ecotoxicology

CiteScore

15.40

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0.00%

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