The first molecular characterization and phylogenetic analysis of bovine papular stomatitis virus in Iran

IF 2 3区农林科学 Q4 IMMUNOLOGY

Comparative Immunology Microbiology and Infectious Diseases Pub Date : 2023-10-24 DOI:10.1016/j.cimid.2023.102081

Hamid Akbari , Mohammadreza Ghorani , Hossein Farhadi , Mehdi Fattahi

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引用次数: 0

Abstract

Bovine papular stomatitis (BPS) generally is a mild viral disease caused by the Bovine papular stomatitis virus (BPSV) belonging to the genus Parapoxvirus (PPV). This study aimed to perform the first molecular characterization and phylogenetic analysis of BPSV in beef calves in Iran. Clinical examinations were carried out on four beef cattle herds in West Azerbaijan province, which had experienced outbreaks of papular lesions. Fifty swab samples were collected from the papular lesions on the muzzle, lips, and oral cavity of affected calves, and after viral DNA extraction, they were subjected to polymerase chain reaction (PCR). The results of PCR confirmed the presence of BPSV in all calves with clinical symptoms. The partial B2L gene was sequenced based on nucleotides, and phylogenetic analysis was performed using MEGA 11.0.13. The analysis revealed that BPSV isolates from beef calves in Iran formed two clades. Clade 1 exhibited similarities to the isolates from Finland, Japan, and Georgia, while Clade 2 was similar to the Zambian isolates. These findings indicate the presence of genetic diversity and potential variability within the virus population. Further studies with larger sample sizes and diverse geographic regions will increase the resolution of the phylogenetic tree and contribute to a comprehensive understanding of how BPSV circulates in the country.

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伊朗牛丘疹性口炎病毒的首次分子鉴定和系统发育分析

牛丘疹性口炎(BPS)通常是由牛丘疹性口炎病毒(BPSV)引起的一种轻度病毒性疾病，属于副痘病毒(PPV)属。本研究旨在进行伊朗牛肉小牛BPSV的首次分子鉴定和系统发育分析。对西阿塞拜疆省爆发丘疹性病变的4个肉牛群进行了临床检查。从患病犊牛的口部、唇部和口腔丘疹病变处采集50份拭子样本，提取病毒DNA后进行聚合酶链反应(PCR)。PCR结果证实所有有临床症状的小牛都存在BPSV。对B2L部分基因进行核苷酸测序，并用MEGA 11.0.13软件进行系统发育分析。分析显示，从伊朗肉牛分离的BPSV形成两个分支。进化枝1与来自芬兰、日本和格鲁吉亚的分离株相似，而进化枝2与来自赞比亚的分离株相似。这些发现表明在病毒种群中存在遗传多样性和潜在变异性。更大样本量和不同地理区域的进一步研究将提高系统发育树的分辨率，并有助于全面了解BPSV如何在该国传播。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Comparative Immunology Microbiology and Infectious Diseases 医学-免疫学

CiteScore

4.60

自引率

0.00%

发文量

102

审稿时长

40 days

期刊介绍： Comparative Immunology, Microbiology & Infectious Diseases aims to respond to the concept of "One Medicine" and to provide a venue for scientific exchange. Based on the concept of "Comparative Medicine" interdisciplinary cooperation between specialists in human and animal medicine is of mutual interest and benefit. Therefore, there is need to combine the respective interest of physicians, veterinarians and other health professionals for comparative studies relevant to either human or animal medicine . The journal is open to subjects of common interest related to the immunology, immunopathology, microbiology, parasitology and epidemiology of human and animal infectious diseases, especially zoonotic infections, and animal models of human infectious diseases. The role of environmental factors in disease emergence is emphasized. CIMID is mainly focusing on applied veterinary and human medicine rather than on fundamental experimental research.