Molecular Prevalence of Larval Stages of Fasciola hepatica in Lymnaea stagnalis Species Snails in the Vicinity of the Ağrı Province

Q3 Medicine

Turkiye parazitolojii dergisi Pub Date : 2023-03-02 DOI:10.4274/tpd.galenos.2022.52714

Ahmet Hakan Ünlü, Rahmi Yıldız, Selahattin Aydemir, Abdurrahman Ekici

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Abstract

Objective: Lymnaea stagnalis known as the great pond snail, is one of the intermediate hosts of Fasciola hepatica, a zoonotic parasite. In this study, it was aimed to determine the larval forms of F. hepatica by polymerase chain reaction (PCR) in L. stagnalis species snails collected from the vicinity of Ağrı province.

Methods: In this study, 150 L. stagnalis snails were collected from the Ağrı province. The freshwater snails brought to the laboratory were dissected, then their soft tissues were examined under a microscope. DNA extraction was performed on the dissected snails. After DNA extraction, PCR was performed using primers targeting the cytochrome c oxidase subunit 1 gene region.

Results: In the microscopic examination, larval forms of F. hepatica could not be detected. However, it was concluded that two (1.3%) L. stagnalis freshwater snails were infected with the larval forms of F. hepatica in the PCR.

Conclusion: It was determined that L. stagnalis served as an intermediate host to F. hepatica in the study area.

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Ağrı省附近滞海螺中肝片吸虫幼虫期分子流行病学研究

目的:大塘螺是一种人畜共患寄生虫肝片吸虫的中间宿主之一。本研究采用聚合酶链反应(PCR)技术，对Ağrı省附近地区采集的钉螺进行了肝螺旋体幼虫的鉴定。方法:在Ağrı省采集钉螺150只。将送到实验室的淡水蜗牛进行解剖，然后在显微镜下观察它们的软组织。对解剖后的蜗牛进行DNA提取。提取DNA后，利用引物对细胞色素c氧化酶亚基1基因区域进行PCR。结果:显微镜检查未检出肝原丝胞菌幼虫。结果表明，2只(1.3%)停滞螺旋体淡水蜗牛感染了肝螺旋体幼虫。结论:在研究区，停滞乳杆菌是肝单胞菌的中间宿主。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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