Plant biomacromolecule delivery methods in the 21st century.

IF 4.9 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Frontiers in genome editing Pub Date : 2022-10-14 eCollection Date: 2022-01-01 DOI:10.3389/fgeed.2022.1011934
Sachin Rustgi, Salman Naveed, Jonathan Windham, Huan Zhang, Gözde S Demirer
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引用次数: 0

Abstract

The 21st century witnessed a boom in plant genomics and gene characterization studies through RNA interference and site-directed mutagenesis. Specifically, the last 15 years marked a rapid increase in discovering and implementing different genome editing techniques. Methods to deliver gene editing reagents have also attempted to keep pace with the discovery and implementation of gene editing tools in plants. As a result, various transient/stable, quick/lengthy, expensive (requiring specialized equipment)/inexpensive, and versatile/specific (species, developmental stage, or tissue) methods were developed. A brief account of these methods with emphasis on recent developments is provided in this review article. Additionally, the strengths and limitations of each method are listed to allow the reader to select the most appropriate method for their specific studies. Finally, a perspective for future developments and needs in this research area is presented.

Abstract Image

21 世纪的植物生物大分子输送方法。
21 世纪,通过 RNA 干扰和定点突变进行的植物基因组学和基因特征研究蓬勃发展。具体来说,在过去 15 年中,发现和实施不同基因组编辑技术的数量迅速增加。提供基因编辑试剂的方法也试图跟上在植物中发现和实施基因编辑工具的步伐。因此,各种瞬时/稳定、快速/长效、昂贵(需要专用设备)/不昂贵、多功能/特异(物种、发育阶段或组织)的方法应运而生。本综述文章简要介绍了这些方法,并重点介绍了最新进展。此外,文章还列出了每种方法的优势和局限性,以便读者为其具体研究选择最合适的方法。最后,文章对这一研究领域的未来发展和需求进行了展望。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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CiteScore
7.00
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0.00%
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审稿时长
13 weeks
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