Der Nachweis von Hepatitis Be-Antigen mit einem Enzymimmunoassay

Zentralblatt für Bakteriologie. 1. Abt. Originale A, Medizinische Mikrobiologie, Infektionskrankheiten und Parasitologie Pub Date : 1980-08-01 DOI:10.1016/S0172-5599(80)80033-2

W. Lange , K.N. Masihi

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Abstract

An enzyme immunoassay (ELISA) from SEROMED was tested for ist suitability in detecting HBeAg and was compared with a radioimmunoassay (RIA) from ABBOTT and with rheophoresis.

From a total of 931 sera tested, HBeAg could be detected in 313 sera with ELISA, 347 sera with RIA and 22 sera with rheophoresis. When 8 HBeAg containing sera were titrated for end points, RIA gave titers which were 2.3-fold higher than ELISA and 575-fold higher than rheophoresis. The differences between RIA and ELISA were shown to be due to somewhat lower sensitivity of ELISA.

However, when sera from patients with Hepatitis B from various times after the onset of hepatitis (maximally 12 months) were tested, there was only a slight difference in sensitivity between ELISA and RIA.

ELISA along with RIA is considered to be a substantially sensitive test for detection of HBeAG as compared to rheophoresis and can be recommended for routine use.

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肝炎抗凝乳法

对SEROMED的酶免疫分析法(ELISA)检测HBeAg的适用性进行了测试，并与ABBOTT的放射免疫分析法(RIA)和流变法进行了比较。在931份血清中，313份ELISA检测到HBeAg, 347份RIA检测到HBeAg, 22份流变症检测到HBeAg。当对8份含HBeAg的血清进行终点滴定时，RIA给出的滴度比ELISA高2.3倍，比流变法高575倍。RIA和ELISA之间的差异是由于ELISA的敏感性较低。然而，当检测乙型肝炎患者在肝炎发病后不同时间(最多12个月)的血清时，ELISA和RIA之间的敏感性只有轻微差异。与流变学相比，ELISA和RIA被认为是检测HBeAG的一种非常敏感的试验，可以推荐常规使用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Zentralblatt für Bakteriologie. 1. Abt. Originale A, Medizinische Mikrobiologie, Infektionskrankheiten und Parasitologie

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