Interaction of polylysine with soluble components of human erythrocyte membranes

D. Danon , C. Howe, L.T. Lee
{"title":"Interaction of polylysine with soluble components of human erythrocyte membranes","authors":"D. Danon ,&nbsp;C. Howe,&nbsp;L.T. Lee","doi":"10.1016/0926-6534(65)90051-5","DOIUrl":null,"url":null,"abstract":"<div><p></p><ul><li><span>1.</span><span><p>1. The interactions of poly-<span>l</span>-lysine with soluble erythrocyte components and with Collocalia mucoid, a sialoprotein of avian origin, were studied by electrophoresis and immuno-electrophoresis in agar gel.</p></span></li><li><span>2.</span><span><p>2. Polylysine combined with erythrocyte membrane glycoprotein and with Collocalia mucoid to form visible precipitates in agar gel; erythrocyte fractions devoid of glycoprotein failed so to react.</p></span></li><li><span>3.</span><span><p>3. A precipitate of virus receptor substance with polylysine could be quantitatively redissolved by the addition of poly-<span>l</span>-aspartic acid to the mixture. Whether this change in solubility occurred as a result of displacement of polylysine from the glycoprotein or by the formation of soluble complexes could not be established.</p></span></li><li><span>4.</span><span><p>4. Neuraminidase (EC 3.2.1.18) treatment of erythrocyte glycoproteins and Collocalia mucoid greatly reduced or abolished their capacity to precipitate with polylysine.</p></span></li><li><span>5.</span><span><p>5. The findings suggest that <em>N</em>-acetylneuraminic acid in the acid glycoprotein of erythrocyte membranes is an important factor in the agglutination of erythrocytes by polybases, a phenomenon thought to have some bearing on intravascular thrombus formation.</p></span></li></ul></div>","PeriodicalId":100163,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Mucoproteins and Mucopolysaccharides","volume":"101 2","pages":"Pages 201-213"},"PeriodicalIF":0.0000,"publicationDate":"1965-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6534(65)90051-5","citationCount":"32","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biochimica et Biophysica Acta (BBA) - Mucoproteins and Mucopolysaccharides","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/0926653465900515","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 32

Abstract

  • 1.

    1. The interactions of poly-l-lysine with soluble erythrocyte components and with Collocalia mucoid, a sialoprotein of avian origin, were studied by electrophoresis and immuno-electrophoresis in agar gel.

  • 2.

    2. Polylysine combined with erythrocyte membrane glycoprotein and with Collocalia mucoid to form visible precipitates in agar gel; erythrocyte fractions devoid of glycoprotein failed so to react.

  • 3.

    3. A precipitate of virus receptor substance with polylysine could be quantitatively redissolved by the addition of poly-l-aspartic acid to the mixture. Whether this change in solubility occurred as a result of displacement of polylysine from the glycoprotein or by the formation of soluble complexes could not be established.

  • 4.

    4. Neuraminidase (EC 3.2.1.18) treatment of erythrocyte glycoproteins and Collocalia mucoid greatly reduced or abolished their capacity to precipitate with polylysine.

  • 5.

    5. The findings suggest that N-acetylneuraminic acid in the acid glycoprotein of erythrocyte membranes is an important factor in the agglutination of erythrocytes by polybases, a phenomenon thought to have some bearing on intravascular thrombus formation.

聚赖氨酸与人红细胞膜可溶性组分的相互作用
1.1. 用琼脂凝胶电泳和免疫电泳研究了聚赖氨酸与可溶性红细胞组分和禽源性唾液蛋白Collocalia mucoid的相互作用。在琼脂凝胶中,聚赖氨酸与红细胞膜糖蛋白和结肠黏液结合形成可见的沉淀物;缺乏糖蛋白的红细胞部分也没有反应。通过在混合物中加入聚l-天冬氨酸,可以定量地再溶解含有聚赖氨酸的病毒受体物质沉淀物。这种溶解度的变化是由于糖蛋白中聚赖氨酸的取代还是由于可溶性复合物的形成而发生的,目前还不能确定。神经氨酸酶(EC 3.2.1.18)对红细胞糖蛋白和结肠黏液的处理大大降低或消除了它们与聚赖氨酸沉淀的能力。研究结果表明,红细胞膜酸性糖蛋白中的n -乙酰神经氨酸是红细胞被多碱基凝集的重要因素,这种现象被认为与血管内血栓形成有一定关系。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信