Treatment of mandibular atrophy by an equine bone substitute: an immunohistochemical study in man

Italian Oral Surgery Pub Date : 2012-12-01 DOI:10.1016/j.ios.2012.03.008

L. Artese , D.A. Di Stefano , G. Iezzi , M. Piccirilli , S. Pagnutti , G. di Gregorio , V. Perrotti

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引用次数: 1

Abstract

Objective

To perform an immunohistochemical evaluation of microvessel density (MVD), vascular endothelial growth factor (VEGF) and nitric oxide synthase (NOS) expression in mandibular ridge regeneration using an equine-derived bone substitute.

Materials and methods

Ridge augmentations were performed in six patients using equine bone. After a six-month healing period, four bone cores from no augmented (control) and four from augmented sites (test) were retrieved and processed for immunohistochemistry.

Results

Statistically, significant differences were found in the MVD expression between control and test sites (p < 0.05). The high and low intensities of VEGF, NOS1 and NOS3 were mainly expressed in test samples with statistical differences with control (p < 0.05).

Conclusions

Within the limits of the small sample size, the present study indicated that equine bone is able to support neovascularization when used as a grafting material.

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马骨替代物治疗下颌萎缩:一项人体免疫组织化学研究

目的应用免疫组化方法评价马源性骨替代物在下颌嵴再生中的微血管密度(MVD)、血管内皮生长因子(VEGF)和一氧化氮合酶(NOS)的表达。材料与方法对6例患者行马骨嵴增强术。在6个月的愈合期后，取出4个来自未增强部位(对照组)和4个来自增强部位(试验组)的骨核进行免疫组织化学处理。结果对照组与试验点MVD表达差异有统计学意义(p <0.05)。VEGF、NOS1、NOS3的高、低表达强度主要在检测样本中表达，与对照组差异有统计学意义(p <0.05)。结论:在小样本量的限制下，本研究表明马骨作为移植材料能够支持新生血管的形成。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Italian Oral Surgery

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