Sm Faysal Bellah, Md Abdus Salam, S M Saker Billah, Md Rezaul Karim
{"title":"Genetic association in <i>CYP3A4</i> and <i>CYP3A5</i> genes elevate the risk of prostate cancer.","authors":"Sm Faysal Bellah, Md Abdus Salam, S M Saker Billah, Md Rezaul Karim","doi":"10.1080/03014460.2023.2171122","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong><i>CYP3A4</i> and <i>CYP3A5</i> are biologically potential genes responsible for prostate cancer.</p><p><strong>Aim: </strong>We aimed to analyse the expression and association of <i>CYP3A4</i> and <i>CYP3A5</i> genes in prostate cancer.</p><p><strong>Subjects and methods: </strong>Web-based bioinformatics tools were used to assess the association of <i>CYP3A4</i> and <i>CYP3A5</i> genes with prostate cancer risks. A case-control study of 210 prostate cancer cases and 207 controls was also approved to determine the allelic variants of the <i>CYP3A4</i> gene- rs2740574 (<i>CYP3A4*1B</i>) and the variant of <i>CYP3A5</i> gene-rs776746 (<i>CYP3A5*3</i>) using Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP). The risk of prostate cancer was estimated as odds ratio (OR) and 95% confidence interval (CI) using unrestricted logistic regression models.</p><p><strong>Results: </strong>Our <i>in silico</i> data confirmed that both <i>CYP3A4</i> and <i>CYP3A5</i> genes are significantly associated with higher prostate cancer risks. In the case of <i>CYP3A4*1B</i> polymorphism, the heterozygote (*1 A/*1B), mutant (*1B/*1B), and combined heterozygote plus mutant (*1A/*1B+*1B/*1B) genotypes showed 3.52-fold, 3.90-fold, and 3.67-fold increased risk of prostate cancer, respectively. In the case of <i>CYP3A5*3</i> polymorphism, the heterozygote (*1/*3), mutant (*3/*3), and combined (*1/*3+*3/*3) genotypes were found to be significantly associated with 5.11-, 5.49-, and 5.28-fold greater risk of prostate cancer, respectively.</p><p><strong>Conclusion: </strong>Our results indicate that <i>CYP3A4*1B</i> and <i>CYP3A5*3</i> are significantly associated with increased prostate cancer risk.KEY MESSAGESBioinformatics tools were used and concluded that the <i>CYP3A4</i> and <i>CYP3A5</i> genes were significantly associated with the development and progression of prostate cancer.<i>CYP3A4</i> and <i>CYP3A5</i> polymorphisms were significantly associated with an increased risk of prostate cancer.Polymerase Chain Reaction (PCR)-Restriction Fragment Length Polymorphism (RFLP) was used to estimate polymorphisms of prostate cancer progression in the Bangladeshi population.</p>","PeriodicalId":50765,"journal":{"name":"Annals of Human Biology","volume":null,"pages":null},"PeriodicalIF":1.2000,"publicationDate":"2023-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Annals of Human Biology","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1080/03014460.2023.2171122","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"ANTHROPOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
Background: CYP3A4 and CYP3A5 are biologically potential genes responsible for prostate cancer.
Aim: We aimed to analyse the expression and association of CYP3A4 and CYP3A5 genes in prostate cancer.
Subjects and methods: Web-based bioinformatics tools were used to assess the association of CYP3A4 and CYP3A5 genes with prostate cancer risks. A case-control study of 210 prostate cancer cases and 207 controls was also approved to determine the allelic variants of the CYP3A4 gene- rs2740574 (CYP3A4*1B) and the variant of CYP3A5 gene-rs776746 (CYP3A5*3) using Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP). The risk of prostate cancer was estimated as odds ratio (OR) and 95% confidence interval (CI) using unrestricted logistic regression models.
Results: Our in silico data confirmed that both CYP3A4 and CYP3A5 genes are significantly associated with higher prostate cancer risks. In the case of CYP3A4*1B polymorphism, the heterozygote (*1 A/*1B), mutant (*1B/*1B), and combined heterozygote plus mutant (*1A/*1B+*1B/*1B) genotypes showed 3.52-fold, 3.90-fold, and 3.67-fold increased risk of prostate cancer, respectively. In the case of CYP3A5*3 polymorphism, the heterozygote (*1/*3), mutant (*3/*3), and combined (*1/*3+*3/*3) genotypes were found to be significantly associated with 5.11-, 5.49-, and 5.28-fold greater risk of prostate cancer, respectively.
Conclusion: Our results indicate that CYP3A4*1B and CYP3A5*3 are significantly associated with increased prostate cancer risk.KEY MESSAGESBioinformatics tools were used and concluded that the CYP3A4 and CYP3A5 genes were significantly associated with the development and progression of prostate cancer.CYP3A4 and CYP3A5 polymorphisms were significantly associated with an increased risk of prostate cancer.Polymerase Chain Reaction (PCR)-Restriction Fragment Length Polymorphism (RFLP) was used to estimate polymorphisms of prostate cancer progression in the Bangladeshi population.
期刊介绍：
Annals of Human Biology is an international, peer-reviewed journal published six times a year in electronic format. The journal reports investigations on the nature, development and causes of human variation, embracing the disciplines of human growth and development, human genetics, physical and biological anthropology, demography, environmental physiology, ecology, epidemiology and global health and ageing research.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
