Halocatena marina sp. nov., a novel filamentous halophilic archaeon isolated from marine tidal flat and emended description of the genus Halocatena.

IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS
Zhang-Ping Wu, Xi-Wen Zheng, Ya-Ping Sun, Bei-Bei Wang, Jing Hou, Heng-Lin Cui
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引用次数: 2

Abstract

Three novel filamentous halophilic archaea, strains DFN5T, RDMS1, and QDMS1, were isolated from the coastal saline soil samples of the intertidal zones located in different regions of Jiangsu Province, China. The colonies of these strains were pinkish-white due to the presence of white spores. These three strains are extremely halophilic and grew optimally at 35-37 °C and pH 7.0-7.5. Based on 16S rRNA and rpoB' gene analysis, strains DFN5T, RDMS1, and QDMS1 gathered together in phylogenetic trees and then clustered with the current species of the genus Halocatena showing 96.9-97.4% and 82.2-82.5% similarities, respectively. Both the 16S rRNA gene-based and rpoB' gene-based phylogenies were fully supported by the phylogenomic analysis, and the overall genome-related indexes indicated that strains DFN5T, RDMS1, and QDMS1 should be a novel species of the genus Halocatena. Genome mining revealed that there are considerable differences in the genes related to β-carotene synthesis among these three strains and the current species of Halocatena. The major polar lipids of strains DFN5T, RDMS1, and QDMS1 are PA, PG, PGP-Me, S-TGD-1, TGD-1, and TGD-2. The minor polar lipids, S-DGD-1, DGD-1, S2-DGD, and S-TeGD may be detected. According to the phenotypic characteristics, phylogenetic analysis, genomic and chemotaxonomic features, strains DFN5T (= CGMCC 1.19401 T = JCM 35422 T), RDMS1 (= CGMCC 1.19411) and QDMS1 (= CGMCC 1.19410) were classified as a novel species of the genus Halocatena with the proposed name, Halocatena marina sp. nov. This is the first report of the description of a novel filamentous haloarchaeon isolated from marine intertidal zones.

Abstract Image

从海洋潮滩中分离出的一种新型丝状嗜盐古菌&对盐藻属的修正描述。
从江苏省不同地区潮间带滨海盐渍土样品中分离到3株新型丝状嗜盐古菌DFN5T、RDMS1和QDMS1。由于存在白色孢子,这些菌株的菌落呈粉白色。这三种菌株都具有极强的嗜盐性,在35-37℃和pH 7.0-7.5的条件下生长最佳。基于16S rRNA和rpoB’基因分析,菌株DFN5T、RDMS1和QDMS1在系统发育树中聚类,与现属Halocatena的相似性分别为969.9% ~ 97.4%和82.2% ~ 82.5%。系统发育分析完全支持基于16S rRNA基因和rpoB’基因的系统发育,总体基因组相关指标表明菌株DFN5T、RDMS1和QDMS1应该是盐菜属的新种。基因组挖掘发现,这3个菌株与现种Halocatena在β-胡萝卜素合成相关基因上存在相当大的差异。菌株DFN5T、RDMS1和QDMS1的主要极性脂质为PA、PG、PGP-Me、S-TGD-1、TGD-1和TGD-2。微量极性脂质,S-DGD-1, DGD-1, S2-DGD, S-TeGD均可检测到。根据表型特征、系统发育分析、基因组学和化学分类特征,将菌株DFN5T (= CGMCC 1.19401 T = JCM 35422 T)、RDMS1 (= CGMCC 1.19411)和QDMS1 (= CGMCC 1.19410)归类为Halocatena属的新种,并命名为Halocatena marina sp. 11。这是首次报道分离自海洋潮间带的一种新型丝状盐古菌。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
ACS Applied Bio Materials
ACS Applied Bio Materials Chemistry-Chemistry (all)
CiteScore
9.40
自引率
2.10%
发文量
464
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