The Isolation and Characterization of Phenolic Compound of Euphorbia Plant/Patikan Cina (Euphorbia thymifolia Linn)

H. Amanda
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Abstract

Euphorbia plant, Euphorbia thymifolia Linn, is one the Euphorbiaceae families that still need to be developed due to its benefits. An effort to be able to use this plant maximumly is by studying the active component in the plant. This study aims to isolate and characterize phenolic compound in euphorbia plant extract using ethyl acetate. 500 gram of euphorbia plant (Patikan Cina) powder was macerated using methanol. Then, the macerated extract was evaporated to eliminate excess solvent and as a result, a solvent-free extract was obtained. Next, the result was partitioned with a solution of methanol : ethyl acetate (1:1) in order to get methanol and ethyl acetate extracts. The ethyl acetate extract of the partition was evaporated to get concentrated ethyl acetate extract. Next, it was isolated in a vacuum liquid and gravitational column chromatography to get pure isolate. Silica gel 60 (0.040 – 0.063 mm) and n-hexane motion phase were used in the separation of concentrated ethyl acetate in vacuum liquid chromatography. There were 16 fractions produced from the yield. Then the fractions were put in TLC. With silica gel 60 (0.2 – 0.5 mm) mesh and n-hexane mobile phase, ethyl acetate gave 5 fractions in gravitational column chromatography and fraction 3 produced a single spot. UV and IR spectroscopy were used to determine the constituents in the isolate. From UV spectrum, max is 268.97 nm. While IR data shows the presence O-H group in 3521.38 cm-1, C – H (alkane) presents at wave number 2926.45 cm-1 reinforced by the appearance of 5 other alkanes absorption at 2857.00 cm-1, 1447.31 cm-1, 1370.18 cm-1, 868.774 cm-1 and 757.887 cm-1 wavelengths. There is also C = O (carbonyl) groups in wave number 1693.19 cm-1. The identification of structures based on UV and IR spectra data has shown that the isolate is a phenolic compound.
大戟属植物(Euphorbia thymifolia Linn)酚类化合物的分离与表征
大戟属植物大戟属植物(Euphorbia thymifolia Linn)是大戟科植物中仍有待开发利用的植物之一。要想最大限度地利用这种植物,就要研究它的活性成分。本研究旨在用乙酸乙酯对大戟提取物中的酚类化合物进行分离鉴定。用甲醇浸泡大戟粉500克。然后,将浸渍过的浸膏蒸发去多余的溶剂,得到无溶剂浸膏。然后用甲醇:乙酸乙酯(1:1)溶液对结果进行分馏,得到甲醇和乙酸乙酯提取物。将隔板的乙酸乙酯萃取物蒸发,得到浓缩乙酸乙酯萃取物。然后在真空液中分离,重力柱层析得到纯分离物。采用硅胶60 (0.040 ~ 0.063 mm)和正己烷运动相进行真空液相色谱分离浓缩乙酸乙酯。从产量中得到16个馏分。然后将馏分放入薄层色谱中。以硅胶60 (0.2 - 0.5 mm)目,正己烷为流动相,乙酸乙酯在重力柱色谱中分为5个部分,第3部分产生一个单点。采用紫外光谱和红外光谱对分离物进行了成分分析。从紫外光谱来看,最大波长为268.97 nm。而红外数据显示,O-H在3521.38 cm-1波段存在,C -H(烷烃)在2926.45 cm-1波段存在,另外5种烷烃在2857.00 cm-1、1447.31 cm-1、1370.18 cm-1、868.774 cm-1和757.887 cm-1波段的吸收增强了C -H的存在。波数1693.19 cm-1中也存在C = O(羰基)基团。根据紫外和红外光谱数据对其结构进行了鉴定,表明该分离物为酚类化合物。
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