Direkter Festphasen-Radioimmunoassay zum Nachweis von Aujeszkyvirus-Antikörpern

Zentralblatt für Bakteriologie. 1. Abt. Originale A, Medizinische Mikrobiologie, Infektionskrankheiten und Parasitologie Pub Date : 1980-06-01 DOI:10.1016/S0172-5599(80)80014-9

Gabriele Döller , Josef Jakubik

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引用次数: 5

Abstract

A direct solid-phase radioimmunoassay (dRIA) was developed in order to demonstrate antibodies against Aujeszky's disease virus (ADV) in sera obtained from pigs and rabbits. In the presence of guinea-pig complement the above test is 160-fold to 1500-fold more sensitive than the neutralization test (NT) and 320-fold to 150000-fold more sensitive than NT without complement. By means of dRIA ADV antibodies could be detected in swine sera obtained from an ADV-infected farm, which were found to be negative in the complement assisted NT. It is possible to test a single dilution of unknown serum by dRIA by comparing same with a standard curve and to make a statement regarding its ADV-specific binding capacity to ¹²⁵I-labelled ADV antigen.

The advantages of dRIA in comparison to the indirect RIA and the advantages and disadvantages with regard to ELISA were discussed.

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可以直接病毒抗体检测

建立了一种直接固相放射免疫测定法(dRIA)，以证明猪和兔血清中抗奥杰斯基病病毒(ADV)的抗体。在豚鼠补体存在的情况下，上述试验比中和试验(NT)敏感性高160- 1500倍，比没有补体的NT敏感性高320- 15万倍。通过dRIA方法，可以从感染ADV农场的猪血清中检测到ADV抗体，这些抗体在补体辅助NT中被发现是阴性的。可以通过将其与标准曲线进行比较，通过dRIA测试单一稀释的未知血清，并对其ADV特异性结合能力作出声明125i标记的ADV抗原。讨论了dRIA与间接RIA相比的优势以及与ELISA相比的优缺点。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Zentralblatt für Bakteriologie. 1. Abt. Originale A, Medizinische Mikrobiologie, Infektionskrankheiten und Parasitologie

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