Isolation and molecular identification of methicillin resistant (MRSA) from different type of wound (Cuts, burn and chemical

Abstract

Three hundred of clinical wound samples (Cuts, burn and chemical) were collected from Murtala Muhammad Specialist Hospital, Kano State. The isolation and identification of was done through culture on Nutrient agar and Mannitol salt agar, Gram staining, microscopic and standard biochemical tests such as (catalase, coagulase, oxidase and hemolysis test) were carried out. Cefoxitin disc diffusion test and molecular analysis was done for the detection of methicillin resistant strain of . A total of one hundred and one isolates were identified in the present study in which 13(12.90%) were MRSA positive and 88(87.10%) were negative MRSA. Polymerase chain reaction (PCR) analysis reveals the presence of mec A gene under 310base pairs nucleotide sequence in the positive MRSA.