Effect of feeding regime on the metabolism of ochratoxin a during the in vitro incubation in buffered rumen fluid from cows

H. Müller, K. Müller, H. Steingass
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引用次数: 18

Abstract

Pure ochratoxin A (OA) was added to buffered rumen fluid collected from two fisrulated cows and incubated under anaerobic conditions. Both animals were fed six diets containing grass, grass silage or hay, and two different amounts of concentrate consisting of barley and soybean meal. Four incubations per animal and diet were carried out at consecutive days. The concentration of OA declined exponentially to a very low or non‐detectable level under all conditions examined, with half‐lives at 0.51 to 2.76 h. The disappearance of OA was accompanied by the appearance of ochratoxin α (Oα) with an average amount of Oα formed relative to the disappearance of OA near 100%, independent of diet and animal. Based on four incubations per animal and diet the rate of OA disappearance was affected (P < 0.1) by the origin of rumen fluid from two animals, as well as by the type of basic component and amount of concentrate in the diet, with interactions between these factors. The disappearance of OA mostly was accelerated (P<0.1) by replacing grass silage or hay by fresh grass and by increasing the content of concentrate from 10 to 50% of dry matter. It is concluded that the capacity of the rumen to detoxify OA is not limited by the yield of Oa from OA but is strongly dependent on animal and diet.
饲喂方式对奶牛瘤胃缓冲液中赭曲霉毒素a体外培养代谢的影响
将纯赭曲霉毒素A (ochratoxin A, OA)添加到2头泌乳奶牛的瘤胃液中,在厌氧条件下孵育。两种动物分别饲喂六种饲粮,其中包括草、草青贮或干草,以及两种不同数量的由大麦和豆粕组成的浓缩物。每只动物4次孵育,连续d饲喂饲料。在所有条件下,OA的浓度都呈指数下降到非常低或无法检测到的水平,半衰期为0.51 ~ 2.76 h。OA的消失伴随着赭曲霉毒素α (ochratoxin α, Oα)的出现,相对于OA的消失,Oα的平均形成量接近100%,与饲料和动物无关。在每只动物和饲粮进行4次孵育的基础上,OA消失率受2只动物瘤胃液来源、饲粮中基本成分类型和精料量的影响(P < 0.1),且这些因素之间存在相互作用。鲜草代替青贮或干草,将精料含量从10%提高到50%,能加速OA的消失(P<0.1)。由此可见,瘤胃对OA的解毒能力不受OA产量的限制,而与动物和日粮密切相关。
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