Differential gene expression associated with flower development of mango (Mangifera indica L.) varieties with different shelf-life

IF 1 4区生物学 Q4 DEVELOPMENTAL BIOLOGY

Gene Expression Patterns Pub Date : 2023-03-01 DOI:10.1016/j.gep.2022.119301

Nimisha Sharma , Mukesh Shivran , Narendra Singh , Anil Kumar Dubey , Sanjay Kumar Singh , Neha Sharma , Ruchi Gupta , Hatkari Vittal , Bikram Pratap Singh , Amitha Mithra Sevanthi , Nagendra Kumar Singh

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引用次数: 0

Abstract

Mango (Mangifera indica L.) is one of the most important commercial fruit crop grown in many parts of the world. Major challenges affecting mango trade are short shelf-life, high susceptibility to chilling injury, post-harvest diseases and consumer demand for improved fruit quality. The objective of the present study was to reveal the key regulators present in bud and flower tissues during flower development stage, associated with fruit development and affect the shelf-life of the mango fruit. RNA-sequencing of contrasting genotypes having short and long shelf-life, was carried out. Comparative differential expression pathway studies of long shelf-life (Totapuri) and short shelf-life (Bombay Green) mango genotypes revealed a total of 177 highly differentially expressed genes. Out of 177 total genes, 101 genes from endoplasmic reticulum pathway and very few from gibberellins (3) and jasmonic acid (1) pathway were identified. Genes from endoplasmic reticulum pathway like hsp 90, SRC2, DFRA, CHS, BG3 and ASPG1 mainly up regulated in Bombay Green. Uniprotein B9R8D3 also shows up regulation in Bombay Green. Ethylene insensitive pathway gene EIL1 up regulated in Bombay Green. Gene CAD1 from phenylpropanoid pathway mainly up regulated in Bombay Green. A total of 4 SSRs and 227 SNPs were mined from these pathways specific to the shelf-life. Molecular studies of endoplasmic reticulum, phenylpropanoid, ethylene, polygalacturonase and hormone pathways at the time of bud and flower formation revealed key regulators that determine the shelf-life of mango fruit.

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不同保质期芒果品种花发育相关基因的差异表达

芒果（Mangifera indica L.）是世界上许多地区种植的最重要的商业水果作物之一。影响芒果贸易的主要挑战是保质期短、易受冷害、收获后疾病以及消费者对提高水果质量的需求。本研究的目的是揭示在花发育阶段芽和花组织中存在的与果实发育有关并影响芒果果实保质期的关键调节因子。对具有短保质期和长保质期的对照基因型进行RNA测序。对长货架期（Totapuri）和短货架期（Bombay Green）芒果基因型的差异表达途径比较研究显示，共有177个高度差异表达的基因。在177个基因中，鉴定出101个来自内质网途径的基因，很少有来自赤霉素（3）和茉莉酸（1）途径的基因。来自内质网途径的基因如hsp90、SRC2、DFRA、CHS、BG3和ASPG1在Bombay Green中主要上调。单一蛋白B9R8D3在孟买绿中也显示出调节作用。乙烯不敏感通路基因EIL1在Bombay Green上调。苯丙素途径的基因CAD1在Bombay Green中主要上调。从这些特定于保质期的途径中总共提取了4个SSR和227个SNP。对芽和花形成时内质网、苯丙烷、乙烯、多聚半乳糖醛酸酶和激素途径的分子研究揭示了决定芒果货架期的关键调节因子。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Gene Expression Patterns 生物-发育生物学

CiteScore

2.30

自引率

0.00%

发文量

审稿时长

35 days

期刊介绍： Gene Expression Patterns is devoted to the rapid publication of high quality studies of gene expression in development. Studies using cell culture are also suitable if clearly relevant to development, e.g., analysis of key regulatory genes or of gene sets in the maintenance or differentiation of stem cells. Key areas of interest include: -In-situ studies such as expression patterns of important or interesting genes at all levels, including transcription and protein expression -Temporal studies of large gene sets during development -Transgenic studies to study cell lineage in tissue formation