An evaluation of the effects on the ovaries of hyperbaric oxygen therapy in a rat model of premature ovarian failure created with cyclophosphamide.

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2023-03-10 DOI:10.4274/tjod.galenos.2023.47817

Fulya Çağlı, Mehmet Akif Baktır, Mehmet Dolanbay, Esra Balcıoğlu, Ahmet Cumaoğlu, Mustafa Ermiş, Enes Karaman, Betül Yalçın, Ercan Mustafa Aygen

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引用次数: 0

Abstract

Objective: To evaluate hyperbaric oxygen therapy (HBO) based on ovarian histology, total antioxidant status (TAS), total oxidant status (TOS), and anti-müllerian hormone (AMH), in the ovarian insufiency (POI) model created with cyclophosphamide (CYP).

Materials and methods: The rats were separated into 3 groups of the control group (n=6), the CYP group (n=6), and the CYP+HBO group (n=6). The rats in the CYP group and the CYP+HBO group were injected intraperitoneally with 200 mg/kg CYP on day 1, followed by 8 mg/kg/day for 14 days to create POI. From the 15^th day onwards, the rats in the CYP+HBO group were placed in a hyperbaric cabin and exposed to 100% oxygen at 2.4 atm pressure for one h, and were then returned to their cages at the end of the hour.

Results: A statistically significant decrease was determined in the primordial and primary follicle counts in the CYP group compared with the control group (p<0.05). In the CYP+HBO group, a statistically significant increase was determined in the primordial and primary follicle counts (p<0.05). The serum AMH levels were seen to be significantly decreased in the CYP group compared with both the control group and the CYP+HBO groups. The HBO was seen to decrease TOS and increase TAS.

Conclusion: HBO could be an alternative treatment to minimize the effect of ovarian follicle loss caused by CYP, which is used for treating tumors that commonly occur in young females of reproductive age.

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高压氧治疗对环磷酰胺引起的卵巢早衰大鼠模型卵巢影响的评价。

目的:评价高压氧治疗(HBO)对卵巢组织学、总抗氧化状态(TAS)、总氧化状态(TOS)和抗勒氏激素(AMH)在环磷酰胺(CYP)所致卵巢功能不全(POI)模型中的作用。材料与方法:将大鼠分为3组:对照组(n=6)、CYP组(n=6)、CYP+HBO组(n=6)。CYP组和CYP+HBO组大鼠在第1天腹腔注射200 mg/kg CYP，随后连续注射8 mg/kg/d，连续14 d形成POI。从第15天起，将CYP+HBO组大鼠置于高压舱中，在2.4 atm压力下接触100%氧气1小时，1小时后返回笼中。结果:与对照组相比，CYP组的原始和原发性卵泡计数有统计学意义的下降(p)结论:HBO可作为一种替代治疗方法，以尽量减少CYP引起的卵巢卵泡丧失的影响，用于治疗常见于育龄年轻女性的肿瘤。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.