Advances and challenges in intravital imaging of craniofacial and dental progenitor cells

IF 2.4 4区生物学 Q2 DEVELOPMENTAL BIOLOGY

genesis Pub Date : 2022-08-18 DOI:10.1002/dvg.23498

Dongwook Yang, Laura Ortinau, Youngjae Jeong, Dongsu Park

引用次数: 1

Abstract

Craniofacial and appendicular bone homeostasis is dynamically regulated by a balance between bone formation and resorption by osteoblasts and osteoclasts, respectively. Despite the developments in multiple imaging techniques in bone biology, there are still technical challenges and limitations in the investigation of spatial/anatomical location of rare stem/progenitor cells and their molecular regulation in tooth and craniofacial bones of living animals. Recent advances in live animal imaging techniques for the craniofacial and dental apparatus can provide new insights in real time into bone stem/progenitor cell dynamics and function in vivo. Here, we review the current inventions and applications of the noninvasive intravital imaging technique and its practical uses and limitations in the analysis of stem/progenitor cells in craniofacial and dental apparatus in vivo. Furthermore, we also explore the potential applications of intravital microscopy in the dental field.

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颅面和牙齿祖细胞活体成像的进展和挑战

颅面骨和阑尾骨的动态平衡分别由成骨细胞和破骨细胞在骨形成和骨吸收之间的平衡来调节。尽管骨生物学的多种成像技术得到了发展，但在研究活体动物牙齿和颅面骨中稀有干细胞/祖细胞的空间/解剖位置及其分子调控方面仍存在技术挑战和局限性。颅面和牙科器械活体成像技术的最新进展可以为实时了解骨干/祖细胞的动态和体内功能提供新的见解。本文综述了无创活体成像技术的最新发明和应用，以及该技术在颅面和牙器官干细胞/祖细胞体内分析中的实际应用和局限性。此外，我们还探讨了活体显微技术在牙科领域的潜在应用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

genesis 生物-发育生物学

CiteScore

3.60

自引率

0.00%

发文量

审稿时长

6-12 weeks

期刊介绍： As of January 2000, Developmental Genetics was renamed and relaunched as genesis: The Journal of Genetics and Development, with a new scope and Editorial Board. The journal focuses on work that addresses the genetics of development and the fundamental mechanisms of embryological processes in animals and plants. With increased awareness of the interplay between genetics and evolutionary change, particularly during developmental processes, we encourage submission of manuscripts from all ecological niches. The expanded numbers of genomes for which sequencing is being completed will facilitate genetic and genomic examination of developmental issues, even if the model system does not fit the “classical genetic” mold. Therefore, we encourage submission of manuscripts from all species. Other areas of particular interest include: 1) the roles of epigenetics, microRNAs and environment on developmental processes; 2) genome-wide studies; 3) novel imaging techniques for the study of gene expression and cellular function; 4) comparative genetics and genomics and 5) animal models of human genetic and developmental disorders. genesis presents reviews, full research articles, short research letters, and state-of-the-art technology reports that promote an understanding of the function of genes and the roles they play in complex developmental processes.