Simple and Rapid Analysis of Penicillins in Milk by HPLC Using Multidimensional: On-line Clean-up and Post-column Photolysis with UV Detection

Abstract

The method was established by using C18 solid-phase extraction, on-line clean-up and post-column photochemical derivatization HPLC with UV detection at 310nm. Penicillins, having UV absorption maxima less than 230nm, were converted quantitatively to molecules having 310nm absorption maxima by photochemical degration. Recoveries at a level of 0.004mg/L were in the range from 87 to 110%. The quantitation limit was 0.002 for penicillin G, oxacillin, cloxacillin and dicloxacillin and 0.004mg/L for nafcillin.