Description of Dryocola gen. nov. and two novel species, Dryocola boscaweniae sp. nov. and Dryocola clanedunensis sp. nov. isolated from the rhizosphere of native British oaks

IF 3.3 2区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Systematic and applied microbiology Pub Date : 2023-04-01 DOI:10.1016/j.syapm.2023.126399

Daniel Maddock , Carrie Brady , Sandra Denman , Dawn Arnold

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引用次数: 1

Abstract

While investigating the role of the rhizosphere in the development of Acute Oak Decline, bacterial strains belonging to the family Enterobacteriaceae were isolated from rhizosphere soil following enrichment for the Enterobacterales. Partial sequencing of several housekeeping genes showed that these strains could not be assigned to an existing genus. Overall, 16 strains were investigated using a polyphasic approach to determine their taxonomic status. This involved phenotypic testing and fatty acid analysis paired with phylogenetic analyses of 16S rRNA and housekeeping gene sequences, as well as phylogenomic analysis of whole genome sequences. Phylogenomic and phylogenetic analyses consistently demonstrated that the 16 isolates could be separated into two distinct clusters in a monophyletic clade situated between the genera Cedecea and Buttiauxella. The two clusters could be genotypically and phenotypically differentiated from each other and from their closest neighbours. As such we propose the description of Dryocola boscaweniae gen. nov. sp. nov. (type strain H6W4^T = CCUG 76177^T = LMG 32610^T) and Dryocola clanedunesis sp. nov. (type strain H11S18^T = CCUG 76181^T = LMG 32611^T).

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英国本土栎树根际分离的杉木栎及两新种——bosscaweniae sp. 11和cledunensis sp. 11的描述

在研究根际在橡树急性衰退发展中的作用时，在对肠杆菌类进行富集后，从根际土壤中分离出属于肠杆菌科的菌株。对几个持家基因的部分测序表明，这些菌株不能被分配到现有的属中。总体而言，使用多相方法对16个菌株进行了研究，以确定它们的分类地位。这涉及表型测试和脂肪酸分析，以及16S rRNA和管家基因序列的系统发育分析，以及全基因组序列的系统进化分析。系统发育基因组和系统发育分析一致表明，这16个分离株可以在Cedecea属和Buttiauxella属之间的单系分支中分离成两个不同的簇。这两个聚类可以从基因型和表型上相互区分，也可以从最接近的邻居区分。因此，我们提出了Dryocola boscaweniae gen.nov.sp.nov.（型菌株H6W4T=CCUG 76177T=LMG 32610T）和Dryocol clanedunesis sp.nov.（型毒株H11S18T=CCUG 76181T=LMG 326 11T）的描述。

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来源期刊

Systematic and applied microbiology 生物-生物工程与应用微生物

CiteScore

7.50

自引率

5.90%

发文量

审稿时长

22 days

期刊介绍： Systematic and Applied Microbiology deals with various aspects of microbial diversity and systematics of prokaryotes. It focuses on Bacteria and Archaea; eukaryotic microorganisms will only be considered in rare cases. The journal perceives a broad understanding of microbial diversity and encourages the submission of manuscripts from the following branches of microbiology: