The Roles of Aerobic Exercise and Folate Supplementation in Hyperhomocysteinemia-Accelerated Atherosclerosis.

IF 1.8 4区医学 Q3 CARDIAC & CARDIOVASCULAR SYSTEMS

Acta Cardiologica Sinica Pub Date : 2023-03-01 DOI:10.6515/ACS.202303_39(2).20221027A

Xingming Zhong, Rong He, Shaohua You, Bo Liu, Xiujie Wang, Jieming Mao

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引用次数: 1

Abstract

Background: Hyperhomocysteinemia (HHcy) is an independent risk factor for atherosclerosis. Effective interventions to reduce HHcy-accelerated atherosclerosis are required.

Objectives: This study aimed to investigate the effects of aerobic exercise (AE) and folate (FA) supplementation on plasma homocysteine (Hcy) level and atherosclerosis development in a mouse model.

Methods: Six-week-old female apoE-/- mice were grouped into five groups (N = 6-8): HHcy (1.8 g/L DL-homocysteine (DL-Hcy) in drinking water), HHcy + AE (1.8 g/L DL-Hcy and aerobic exercise training on a treadmill), HHcy + FA (1.8 g/L DL-Hcy and 0.006% folate in diet), HHcy + AE + FA (1.8 g/L DL-Hcy, 0.006% folate, and aerobic exercise training on a treadmill), and a control group (regular water and diet). All treatment was sustained for 8 weeks. Triglyceride, cholesterol, lipoprotein, and Hcy levels were determined enzymatically. Plaque and monocyte chemoattractant protein-1 (MCP-1) expression levels in mouse aortic roots were evaluated by immunohistochemistry.

Results: Compared to the HHcy group (18.88 ± 6.13 μmol/L), plasma Hcy concentration was significantly reduced in the HHcy + AE (14.79 ± 3.05 μmol/L, p = 0.04), HHcy + FA (9.4 ± 3.85 μmol/L, p < 0.001), and HHcy + AE + FA (9.33 ± 2.21 μmol/L, p < 0.001) groups. Significantly decreased aortic root plaque area and plaque burden were found in the HHcy + AE and HHcy + AE + FA groups compared to those in the HHcy group (both p < 0.05). Plasma MCP-1 level and MCP-1 expression in atherosclerotic lesions were significantly decreased in the HHcy + AE and HHcy + AE + FA groups compared to the HHcy group (all p < 0.05).

Conclusions: AE reduced atherosclerosis development in HHcy apoE-/- mice independently of reducing Hcy levels. FA supplementation decreased plasma Hcy levels without attenuating HHcy-accelerated atherosclerosis. AE and FA supplementation have distinct mechanisms in benefiting atherosclerosis.

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有氧运动和叶酸补充在高同型半胱氨酸血症加速动脉粥样硬化中的作用。

背景:高同型半胱氨酸血症(HHcy)是动脉粥样硬化的独立危险因素。我们需要有效的干预措施来减少hcy加速的动脉粥样硬化。目的:探讨有氧运动(AE)和叶酸(FA)补充对小鼠血浆同型半胱氨酸(Hcy)水平和动脉粥样硬化发展的影响。方法:6周龄雌性apoE-/-小鼠分为5组(N = 6-8): HHcy组(饮水1.8 g/L dl -同型半胱氨酸(DL-Hcy))、HHcy + AE组(1.8 g/L DL-Hcy +跑步机有氧运动训练)、HHcy + FA组(饮食1.8 g/L DL-Hcy + 0.006%叶酸)、HHcy + AE + FA组(饮食1.8 g/L DL-Hcy + 0.006%叶酸+跑步机有氧运动训练)和对照组(正常饮水和饮食)。所有治疗均持续8周。用酶法测定甘油三酯、胆固醇、脂蛋白和Hcy水平。应用免疫组织化学方法检测小鼠主动脉根部斑块和单核细胞趋化蛋白-1 (MCP-1)的表达水平。结果:与HHcy组(18.88±6.13 μmol/L)相比，HHcy + AE组(14.79±3.05 μmol/L, p = 0.04)、HHcy + FA组(9.4±3.85 μmol/L, p < 0.001)、HHcy + AE + FA组(9.33±2.21 μmol/L, p < 0.001)血浆Hcy浓度显著降低。与HHcy组相比，HHcy + AE组和HHcy + AE + FA组主动脉根部斑块面积和斑块负担均显著减少(p < 0.05)。与HHcy组相比，HHcy + AE组和HHcy + AE + FA组血浆MCP-1水平及动脉粥样硬化病变中MCP-1表达均显著降低(均p < 0.05)。结论:AE降低了HHcy apoE-/-小鼠动脉粥样硬化的发展，独立于降低Hcy水平。补充FA降低了血浆Hcy水平，但没有减轻Hcy加速的动脉粥样硬化。AE和FA的补充对动脉粥样硬化有不同的益处机制。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Acta Cardiologica Sinica 医学-心血管系统

CiteScore

2.90

自引率

15.80%

发文量

144

审稿时长

>12 weeks

期刊介绍： Acta Cardiologica Sinica welcomes all the papers in the fields related to cardiovascular medicine including basic research, vascular biology, clinical pharmacology, clinical trial, critical care medicine, coronary artery disease, interventional cardiology, arrythmia and electrophysiology, atherosclerosis, hypertension, cardiomyopathy and heart failure, valvular and structure cardiac disease, pediatric cardiology, cardiovascular surgery, and so on. We received papers from more than 20 countries and areas of the world. Currently, 40% of the papers were submitted to Acta Cardiologica Sinica from Taiwan, 20% from China, and 20% from the other countries and areas in the world. The acceptance rate for publication was around 50% in general.