{"title":"Evaluation of microscopy and PCR for detection of <i>Dientamoeba fragilis</i>.","authors":"Mona Mohamed Tolba, Amal Farahat Allam, Safia Saleh Khalil, Wagdy Makram Elshouki, Amel Youssef Shehab","doi":"10.4103/tp.tp_93_21","DOIUrl":null,"url":null,"abstract":"<p><strong>Introduction: </strong><i>Dientamoeba fragilis</i> (<i>D. fragilis</i>) diagnosis is an intestinal protozoan parasite globally found in rural and urban areas and is attracting a growing interest. Its prevalence in stool varies from 0.2% to more than 19% depending upon the population studied.</p><p><strong>Materials and methods: </strong>This study was based on the examination of 100 stool samples of randomly referred cases in a rural area in Motobus district, Kafr El-Sheikh governorate, Egypt. Our aim was to investigate the presence of <i>D. fragilis</i> in stool of the examined individuals using conventional polymerase chain reaction (PCR) compared to wet mount and trichrome stain with confirmation of infection by transmission electron microscopy.</p><p><strong>Results: </strong><i>D. fragilis</i> was detected in 13/100 of the stool samples examined using wet mount smears, while trichrome stain detected 17/100. Conventional PCR diagnosed 41 cases of <i>D. fragilis</i> in the studied group. A very good agreement was found between wet mount and trichrome stain for diagnosing <i>D. fragilis</i>, while there was fair agreement between conventional PCR and both microscopy methods. Transmission electron microscope was performed on pooled positive samples that revealed the internal structures of <i>D. fragilis</i> trophozoite with its characteristic nucleus.</p><p><strong>Conclusions: </strong>PCR technique was superior to microscopy for the detection of <i>D. fragilis</i>. Trichrome stain remains vital for microscopic diagnosis.</p>","PeriodicalId":37825,"journal":{"name":"Tropical Parasitology","volume":"12 2","pages":"87-93"},"PeriodicalIF":0.0000,"publicationDate":"2022-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9832490/pdf/","citationCount":"3","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Tropical Parasitology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.4103/tp.tp_93_21","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2022/11/24 0:00:00","PubModel":"Epub","JCR":"Q3","JCRName":"Medicine","Score":null,"Total":0}
引用次数: 3
Abstract
Introduction: Dientamoeba fragilis (D. fragilis) diagnosis is an intestinal protozoan parasite globally found in rural and urban areas and is attracting a growing interest. Its prevalence in stool varies from 0.2% to more than 19% depending upon the population studied.
Materials and methods: This study was based on the examination of 100 stool samples of randomly referred cases in a rural area in Motobus district, Kafr El-Sheikh governorate, Egypt. Our aim was to investigate the presence of D. fragilis in stool of the examined individuals using conventional polymerase chain reaction (PCR) compared to wet mount and trichrome stain with confirmation of infection by transmission electron microscopy.
Results: D. fragilis was detected in 13/100 of the stool samples examined using wet mount smears, while trichrome stain detected 17/100. Conventional PCR diagnosed 41 cases of D. fragilis in the studied group. A very good agreement was found between wet mount and trichrome stain for diagnosing D. fragilis, while there was fair agreement between conventional PCR and both microscopy methods. Transmission electron microscope was performed on pooled positive samples that revealed the internal structures of D. fragilis trophozoite with its characteristic nucleus.
Conclusions: PCR technique was superior to microscopy for the detection of D. fragilis. Trichrome stain remains vital for microscopic diagnosis.
简介：脆弱Dientamoeba fragilis（D.fragilis）是一种在全球农村和城市地区发现的肠道原生动物寄生虫，引起了人们越来越多的兴趣。根据研究人群的不同，其在粪便中的患病率从0.2%到19%不等。材料和方法：本研究基于对埃及Kafr El Sheikh省Motobus区一个农村地区随机转诊病例的100份粪便样本的检查。我们的目的是使用常规聚合酶链式反应（PCR）与湿染和三色染色相比较，研究受试者粪便中是否存在脆弱D.fragilis，并通过透射电子显微镜确认感染。结果：在湿涂片检查的粪便样品中，有13/100份检出脆弱D.fragilis，而三色染色检出17/100份。常规聚合酶链式反应在研究组中诊断出41例脆弱D.fragilis。湿悬法和三色染色法在诊断脆弱D.fragilis方面有很好的一致性，而常规PCR和两种显微镜方法之间有相当的一致性。对合并的阳性样品进行透射电子显微镜检查，揭示了脆弱D.fragilis滋养体的内部结构及其特征性细胞核。结论：聚合酶链式反应检测脆弱D.fragilis的效果优于显微镜。三色染色对显微镜诊断仍然至关重要。
期刊介绍：
Tropical Parasitology, a publication of Indian Academy of Tropical Parasitology, is a peer-reviewed online journal with Semiannual print on demand compilation of issues published. The journal’s full text is available online at www.tropicalparasitology.org. The journal allows free access (Open Access) to its contents and permits authors to self-archive final accepted version of the articles on any OAI-compliant institutional / subject-based repository. The journal will cover technical and clinical studies related to health, ethical and social issues in field of parasitology. Articles with clinical interest and implications will be given preference.
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