Natronogracilivirga saccharolytica gen. nov., sp. nov. and Cyclonatronum proteinivorum gen. nov., sp. nov., haloalkaliphilic organotrophic bacteroidetes from hypersaline soda lakes forming a new family Cyclonatronaceae fam. nov. in the order Balneolales
IF 4.3 3区 材料科学Q1 ENGINEERING, ELECTRICAL & ELECTRONIC
Tatjana N. Zhilina , Dimitry Y. Sorokin , Stepan V. Toshchakov , Ilya V. Kublanov , Daria G. Zavarzina
{"title":"Natronogracilivirga saccharolytica gen. nov., sp. nov. and Cyclonatronum proteinivorum gen. nov., sp. nov., haloalkaliphilic organotrophic bacteroidetes from hypersaline soda lakes forming a new family Cyclonatronaceae fam. nov. in the order Balneolales","authors":"Tatjana N. Zhilina , Dimitry Y. Sorokin , Stepan V. Toshchakov , Ilya V. Kublanov , Daria G. Zavarzina","doi":"10.1016/j.syapm.2023.126403","DOIUrl":null,"url":null,"abstract":"<div><p><span><span>Two heterotrophic bacteroidetes strains were isolated as satellites from autotrophic enrichments inoculated with samples from hypersaline soda lakes in southwestern </span>Siberia. Strain Z-1702</span><sup>T</sup><span> is an obligate anaerobic fermentative saccharolytic bacterium from an iron-reducing enrichment culture, while </span><em>Ca</em>. Cyclonatronum proteinivorum Omega<sup>T</sup><span> is an obligate aerobic proteolytic microorganism<span> from a cyanobacterial enrichment. Cells of isolated bacteria are characterized by highly variable morphology. Both strains are chloride-independent moderate salt-tolerant obligate alkaliphiles<span> and mesophiles. Strain Z-1702</span></span></span><sup>T</sup><span><span><span> ferments glucose, maltose, fructose, </span>mannose, </span>sorbose<span>, galactose<span>, cellobiose, </span></span></span><em>N</em><span><span>-acetyl-glucosamine and alpha-glucans, including starch, glycogen, dextrin, and </span>pullulan. Strain Omega</span><sup>T</sup><span> is strictly proteolytic utilizing a range of proteins and peptones<span>. The main polar lipid fatty acid in both strains is iso-C</span></span><sub>15:0</sub>, while other major components are various C<sub>16</sub> and C<sub>17</sub><span> isomers. According to pairwise sequence alignments using BLAST </span><em>Gracilimonas</em><span> was the nearest cultured relative to both strains (<90% of 16S rRNA gene sequence identity). Phylogenetic analysis placed strain Z-1702</span><sup>T</sup> and strain Omega<sup>T</sup> as two different genera in a deep-branching clade of the new family level within the order <em>Balneolales</em> with genus. Based on physiological characteristics and phylogenetic position of strain Z-1702<sup>T</sup> it was proposed to represent a novel genus and species <em>Natronogracilivirga saccharolityca</em> gen. nov., sp. nov. (= DSMZ 109061<sup>T</sup> =JCM 32930<sup>T</sup> =VKM B 3262<sup>T</sup>). Furthermore, phylogenetic and phenotypic parameters of <em>N. saccharolityca</em> and <em>C. proteinivorum</em> gen. nov., sp. nov., strain Omega<sup>T</sup> (=JCM 31662<sup>T</sup>, =UNIQEM U979<sup>T</sup>), make it possible to include them into a new family with a proposed designation <em>Cyclonatronaceae</em> fam. nov..</p></div>","PeriodicalId":3,"journal":{"name":"ACS Applied Electronic Materials","volume":null,"pages":null},"PeriodicalIF":4.3000,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"ACS Applied Electronic Materials","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0723202023000127","RegionNum":3,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"ENGINEERING, ELECTRICAL & ELECTRONIC","Score":null,"Total":0}
引用次数: 1
Abstract
Two heterotrophic bacteroidetes strains were isolated as satellites from autotrophic enrichments inoculated with samples from hypersaline soda lakes in southwestern Siberia. Strain Z-1702T is an obligate anaerobic fermentative saccharolytic bacterium from an iron-reducing enrichment culture, while Ca. Cyclonatronum proteinivorum OmegaT is an obligate aerobic proteolytic microorganism from a cyanobacterial enrichment. Cells of isolated bacteria are characterized by highly variable morphology. Both strains are chloride-independent moderate salt-tolerant obligate alkaliphiles and mesophiles. Strain Z-1702T ferments glucose, maltose, fructose, mannose, sorbose, galactose, cellobiose, N-acetyl-glucosamine and alpha-glucans, including starch, glycogen, dextrin, and pullulan. Strain OmegaT is strictly proteolytic utilizing a range of proteins and peptones. The main polar lipid fatty acid in both strains is iso-C15:0, while other major components are various C16 and C17 isomers. According to pairwise sequence alignments using BLAST Gracilimonas was the nearest cultured relative to both strains (<90% of 16S rRNA gene sequence identity). Phylogenetic analysis placed strain Z-1702T and strain OmegaT as two different genera in a deep-branching clade of the new family level within the order Balneolales with genus. Based on physiological characteristics and phylogenetic position of strain Z-1702T it was proposed to represent a novel genus and species Natronogracilivirga saccharolityca gen. nov., sp. nov. (= DSMZ 109061T =JCM 32930T =VKM B 3262T). Furthermore, phylogenetic and phenotypic parameters of N. saccharolityca and C. proteinivorum gen. nov., sp. nov., strain OmegaT (=JCM 31662T, =UNIQEM U979T), make it possible to include them into a new family with a proposed designation Cyclonatronaceae fam. nov..
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