Polyunsaturated fatty acids alter the formation of lipid droplets and eicosanoid production in Leishmania promastigotes.

IF 2.5 4区 医学 Q2 PARASITOLOGY
Yasmin Monara Ferreira de Sousa Andrade, Monara Viera de Castro, Victor de Souza Tavares, Rayane da Silva Oliveira Souza, Lúcia Helena Faccioli, Jonilson Berlink Lima, Carlos Arterio Sorgi, Valéria M Borges, Théo Araújo-Santos
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Abstract

Background: The knowledge about eicosanoid metabolism and lipid droplet (LD) formation in the Leishmania is very limited and new approaches are needed to identify which bioactive molecules are produced of them.

Objectives: Herein, we compared LDs and eicosanoids biogenesis in distinct Leishmania species which are etiologic agents of different clinical forms of leishmaniasis.

Methods: For this, promastigotes of Leishmania amazonensis, L. braziliensis and L. infantum were stimulated with polyunsaturated fatty acids (PUFA) and LD and eicosanoid production was evaluated. We also compared mutations in structural models of human-like cyclooxygenase-2 (GP63) and prostaglandin F synthase (PGFS) proteins, as well as the levels of these enzymes in parasite cell extracts.

Findings: PUFAs modulate the LD formation in L. braziliensis and L. infantum. Leishmania spp with equivalent tissue tropism had same protein mutations in GP63 and PGFS. No differences in GP63 production were observed among Leishmania spp, however PGFS production increased during the parasite differentiation. Stimulation with arachidonic acid resulted in elevated production of hydroxyeicosatetraenoic acids compared to prostaglandins.

Main conclusions: Our data suggest LD formation and eicosanoid production are distinctly modulated by PUFAS dependent of Leishmania species. In addition, eicosanoid-enzyme mutations are more similar between Leishmania species with same host tropism.

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多不饱和脂肪酸改变利什曼原虫脂滴的形成和类二十烷的产生。
背景:关于利什曼原虫类二十烷代谢和脂滴形成的知识非常有限,需要新的方法来确定它们产生哪些生物活性分子。目的:在此,我们比较了不同利什曼原虫种类的ld和二十烷类生物发生,它们是不同临床形式利什曼病的病原。方法:用多不饱和脂肪酸(PUFA)和LD刺激亚马逊利什曼原虫、巴西利什曼原虫和婴儿利什曼原虫的promastigotes,并评价其类二十烷酸的产量。我们还比较了人样环氧化酶-2 (GP63)和前列腺素F合成酶(PGFS)蛋白结构模型的突变,以及寄生虫细胞提取物中这些酶的水平。结果:PUFAs可调节巴西乳杆菌和婴儿乳杆菌的LD形成。具有相同组织趋向性的利什曼原虫在GP63和PGFS中有相同的蛋白突变。在利什曼原虫中,GP63的产量没有差异,但在寄生虫分化过程中,PGFS的产量增加。与前列腺素相比,花生四烯酸刺激导致羟基二十碳四烯酸的产生升高。主要结论:我们的数据表明LD的形成和类二十烷酸的产生明显受到利什曼原虫PUFAS依赖性的调节。此外,具有相同宿主倾向的利什曼原虫种间的类二十烷酶突变更为相似。
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来源期刊
CiteScore
5.00
自引率
3.60%
发文量
91
审稿时长
3-8 weeks
期刊介绍: Memórias do Instituto Oswaldo Cruz is a journal specialized in microbes & their vectors causing human infections. This means that we accept manuscripts covering multidisciplinary approaches and findings in the basic aspects of infectious diseases, e.g. basic in research in prokariotes, eukaryotes, and/or virus. Articles must clearly show what is the main question to be answered, the hypothesis raised, and the contribution given by the study. Priority is given to manuscripts reporting novel mechanisms and general findings concerning the biology of human infectious prokariotes, eukariotes or virus. Papers reporting innovative methods for diagnostics or that advance the basic research with these infectious agents are also welcome. It is important to mention what we do not publish: veterinary infectious agents research, taxonomic analysis and re-description of species, epidemiological studies or surveys or case reports and data re-analysis. Manuscripts that fall in these cases or that are considered of low priority by the journal editorial board, will be returned to the author(s) for submission to another journal.
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