Pharmacological Investigation of Fluoro, Iodo and Hydroxy Derivative of Chloro Substituted Homoisoflavonoids

Abstract

In present study, the synthesized substituted homoisoflavonoid derivatives were screened for their in vivo/in vitro antiarthritic activity, in vitro anti-inflammatory and DPPH free radical scavenging activity. The male Wistar rats were used for investigation of in vivo antiarthritic activity against complete freund’s adjuvant (CFA) induced arthritis and assessment was done for change in paw volume, serum marker enzymes (ALP, SGOT and SGPT) and membrane stabilization potential. in vitro anti-inflammatory activity was assessed by the protein denaturation method. In vitro free radical scavenging activity was assessed by the DPPH method. The result indicated that compound HIFa showed a significant antiarthritic activity as compared to other substituted homoisoflavonoid derivatives. The significant membrane stabilization and inhibition of protein denaturation showed in vitro antiarthritic and antiinflammatory activity of substituted homoisoflavonoid derivatives. The substituted homoisoflavonoid derivatives showed dose dependent DPPH free radical scavenging activity. From the present study, it was observed that the iodo derivative of substituted homoisoflavonoid derivatives have significant pharmacological activities as compared to floro and hydroxyl derivatives.