The InBIO barcoding initiative database: DNA barcodes of Iberian Trichoptera, documenting biodiversity for freshwater biomonitoring in a Mediterranean hotspot.
Joana Pauperio, Luis Martin Gonzalez, Jesus Martinez, Marcos A González, Filipa Ms Martins, Joana Veríssimo, Pamela Puppo, Joana Pinto, Cátia Chaves, Catarina J Pinho, José Manuel Grosso-Silva, Lorenzo Quaglietta, Teresa Luísa L Silva, Pedro Sousa, Paulo Celio Alves, Nuno Fonseca, Pedro Beja, Sónia Ferreira
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引用次数: 0
Abstract
Background: The Trichoptera are an important component of freshwater ecosystems. In the Iberian Peninsula, 380 taxa of caddisflies are known, with nearly 1/3 of the total species being endemic in the region. A reference collection of morphologically identified Trichoptera specimens, representing 142 Iberian taxa, was constructed. The InBIO Barcoding Initiative (IBI) Trichoptera 01 dataset contains records of 438 sequenced specimens. The species of this dataset correspond to about 37% of Iberian Trichoptera species diversity. Specimens were collected between 1975 and 2018 and are deposited in the IBI collection at the CIBIO (Research Center in Biodiversity and Genetic Resources, Portugal) or in the collection Marcos A. González at the University of Santiago de Compostela (Spain).
New information: Twenty-nine species, from nine different families, were new additions to the Barcode of Life Data System (BOLD). A success identification rate of over 80% was achieved when comparing morphological identifications and DNA barcodes for the species analysed. This encouraging step advances incorporation of informed Environmental DNA tools in biomonitoring schemes, given the shortcomings of morphological identifications of larvae and adult Caddisflies in such studies. DNA barcoding was not successful in identifying species in six Trichoptera genera: Hydropsyche (Hydropsychidae), Athripsodes (Leptoceridae), Wormaldia (Philopotamidae), Polycentropus (Polycentropodidae) Rhyacophila (Rhyacophilidae) and Sericostoma (Sericostomatidae). The high levels of intraspecific genetic variability found, combined with a lack of a barcode gap and a challenging morphological identification, rendered these species as needing additional studies to resolve their taxonomy.
期刊介绍:
Founded in 1922, Foreign Affairs is a prominent American magazine that focuses on international relations and U.S. foreign policy. It is published by the Council on Foreign Relations, an esteemed nonpartisan think tank and membership organization dedicated to analyzing U.S. foreign policy and global affairs. While the print magazine is released every two months, the website offers daily articles and publishes anthologies every other month.