Bioinformatic analysis and validation of cardiac hypertrophy-related genes.

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2023-03-01 DOI:10.4149/gpb_2022059

Ai Meng, Siang Wei, Lihuan Yan, Yao Xiao, Zhiwen Ding, Yan Feng

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Abstract

In this study, we have screened genes involved in myocardial hypertrophy (MH) using a mice model for compensatory stress overload (transverse aortic constriction, TAC) and bioinformatics. Microarrays were downloaded, and according to the Venn diagram, three groups of data intersections were obtained. Gene function was analyzed by Gene Ontology (GO) and the Kyoto Encyclopedia of Genes and Genomes (KEGG), whereas protein-protein interactions (PPI) were analyzed using the STRING database. A mouse aortic arch ligation model was established to verify and screen the expression of hub genes. A total of 53 (DEGs) and 32 PPI genes were screened out. GO analysis showed DEGs mainly involved in cytokine and peptide inhibitor activity. KEGG analysis focused on ECM receptor interaction and osteoclast differentiation. Expedia co-expression gene network analysis showed that Serpina3n, Cdkn1a, Fos, Col5a2, Fn1 and Timp1 participated in the occurrence and development of MH. RT-qPCR verified that all the other 9 hub genes except Lox were highly expressed in TAC mice. This study lays a foundation for further study on the molecular mechanism of MH and for screening of molecular markers.

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心脏肥大相关基因的生物信息学分析和验证。

在这项研究中，我们使用代偿性应激过载小鼠模型(横断主动脉收缩，TAC)和生物信息学筛选了涉及心肌肥厚(MH)的基因。下载微阵列，根据维恩图，得到三组数据交集。通过基因本体(GO)和京都基因与基因组百科全书(KEGG)分析基因功能，使用STRING数据库分析蛋白质-蛋白质相互作用(PPI)。建立小鼠主动脉弓结扎模型，验证和筛选中枢基因的表达。共筛选出53个(DEGs)基因和32个PPI基因。氧化石墨烯分析显示，DEGs主要参与细胞因子和肽抑制剂活性。KEGG分析的重点是ECM受体相互作用和破骨细胞分化。Expedia共表达基因网络分析显示，Serpina3n、Cdkn1a、Fos、Col5a2、Fn1和Timp1参与了MH的发生发展，RT-qPCR证实除Lox外，其余9个枢纽基因均在TAC小鼠中高表达。本研究为进一步研究MH的分子机制和分子标记的筛选奠定了基础。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.