Photoprotective effect of solid lipid nanoparticles of rutin against UVB radiation damage on skin biopsies and tissue-engineered skin.

IF 3 4区医学 Q2 CHEMISTRY, APPLIED

Journal of microencapsulation Pub Date : 2022-11-01 DOI:10.1080/02652048.2022.2156631

Rodrigo Molina Martins, Silvia de, Siqueira Martins, Gustavo Luis Ferreira Barbosa, Maria José Vieira Fonseca, Patrick J Rochette, Véronique J Moulin, Luis Alexandre Pedro de Freitas

{"title":"Photoprotective effect of solid lipid nanoparticles of rutin against UVB radiation damage on skin biopsies and tissue-engineered skin.","authors":"Rodrigo Molina Martins, Silvia de, Siqueira Martins, Gustavo Luis Ferreira Barbosa, Maria José Vieira Fonseca, Patrick J Rochette, Véronique J Moulin, Luis Alexandre Pedro de Freitas","doi":"10.1080/02652048.2022.2156631","DOIUrl":null,"url":null,"abstract":"Solid lipid nanoparticles (SLNs) containing rutin were prepared to enhance their photochemopreventive effect on the skin. SLNs were produced by the hot melt microemulsion technique. Two 3D skin models: ex vivo skin explants and 3D tissue engineering skin were used to evaluate the photochemopreventive effect of topical formulations containing rutin SLNs, against ultraviolet B (UVB) radiation, inducing sunburn cells, caspase-3, cyclobutane pyrimidine dimers, lipid peroxidation, and metalloproteinase formation. The rutin SLNs presented average size of 74.22 ± 2.77 nm, polydispersion index of 0.16 ± 0.04, encapsulation efficiency of 98.90 ± 0.25%, and zeta potential of -53.0 ± 1.61 mV. The rutin SLNs were able to efficiently protect against UVB induced in the analysed parameters in both skin models. Furthermore, the rutin SLNs inhibited lipid peroxidation and metalloproteinase formation. These results support the use of rutin SLNs as skin photochemopreventive agents for topical application to the skin.","PeriodicalId":16391,"journal":{"name":"Journal of microencapsulation","volume":"39 7-8","pages":"668-679"},"PeriodicalIF":3.0000,"publicationDate":"2022-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"3","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of microencapsulation","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1080/02652048.2022.2156631","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"CHEMISTRY, APPLIED","Score":null,"Total":0}

引用次数: 3

Abstract

Solid lipid nanoparticles (SLNs) containing rutin were prepared to enhance their photochemopreventive effect on the skin. SLNs were produced by the hot melt microemulsion technique. Two 3D skin models: ex vivo skin explants and 3D tissue engineering skin were used to evaluate the photochemopreventive effect of topical formulations containing rutin SLNs, against ultraviolet B (UVB) radiation, inducing sunburn cells, caspase-3, cyclobutane pyrimidine dimers, lipid peroxidation, and metalloproteinase formation. The rutin SLNs presented average size of 74.22 ± 2.77 nm, polydispersion index of 0.16 ± 0.04, encapsulation efficiency of 98.90 ± 0.25%, and zeta potential of -53.0 ± 1.61 mV. The rutin SLNs were able to efficiently protect against UVB induced in the analysed parameters in both skin models. Furthermore, the rutin SLNs inhibited lipid peroxidation and metalloproteinase formation. These results support the use of rutin SLNs as skin photochemopreventive agents for topical application to the skin.

查看原文本刊更多论文

芦丁固体脂质纳米颗粒对UVB辐射损伤的光保护作用。

制备了含有芦丁的固体脂质纳米颗粒(sln)，以增强其对皮肤的光化学预防作用。采用热熔微乳液法制备了sln。采用体外皮肤外植体和3D组织工程皮肤两种3D皮肤模型，评估含有芦丁sln的外用配方对紫外线B (UVB)辐射、诱导晒伤细胞、caspase-3、环丁烷嘧啶二聚体、脂质过氧化和金属蛋白酶形成的光化学预防作用。制备的芦丁SLNs平均尺寸为74.22±2.77 nm，多色散指数为0.16±0.04，包封效率为98.90±0.25%，zeta电位为-53.0±1.61 mV。芦丁sln在两种皮肤模型中均能有效地抵抗所分析参数诱导的UVB。此外，芦丁sln抑制脂质过氧化和金属蛋白酶的形成。这些结果支持使用芦丁sln作为皮肤光化学预防剂局部应用于皮肤。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Journal of microencapsulation 工程技术-工程：化工

CiteScore

6.30

自引率

2.60%

发文量

审稿时长

3 months

期刊介绍： The Journal of Microencapsulation is a well-established, peer-reviewed journal dedicated to the publication of original research findings related to the preparation, properties and uses of individually encapsulated novel small particles, as well as significant improvements to tried-and-tested techniques relevant to micro and nano particles and their use in a wide variety of industrial, engineering, pharmaceutical, biotechnology and research applications. Its scope extends beyond conventional microcapsules to all other small particulate systems such as self assembling structures that involve preparative manipulation. The journal covers: Chemistry of encapsulation materials Physics of release through the capsule wall and/or desorption from carrier Techniques of preparation, content and storage Many uses to which microcapsules are put.