Modulation of Inula racemosa Hook Extract on Cardioprotection by Ischemic Preconditioning in Hyperlipidaemic Rats.

IF 1.2 Q3 INTEGRATIVE & COMPLEMENTARY MEDICINE

Journal of Pharmacopuncture Pub Date : 2022-12-31 DOI:10.3831/KPI.2022.25.4.369

Arun Kumar Tiwari, Pushpraj S Gupta, Mahesh Prasad, Paraman Malairajan

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Abstract

Objectives: Hyperlipidemia (HL) is a major cause of ischemic heart diseases. The size-limiting effect of ischemic preconditioning (IPC), a cardioprotective phenomenon, is reduced in HL, possibly because of the opening of the mitochondrial permeability transition pore (MPTP). The objective of this study is to see what effect pretreatment with Inula racemosa Hook root extract (IrA) had on IPC-mediated cardioprotection on HL Wistar rat hearts. An isolated rat heart was mounted on the Langendorff heart array, and then ischemia reperfusion (I/R) and IPC cycles were performed. Atractyloside (Atr) is an MPTP opener.

Methods: The animals were divided into ten groups, each consisting of six rats (n = 6), to investigate the modulation of I. racemosa Hook extract on cardioprotection by IPC in HL hearts Sham control, I/R Control, IPC control, I/R + HL, I/R + IrA + HL, IPC + HL, IPC + NS + HL, IPC + IrA+ HL, IPC + Atr + oxidative stress, mitochondrial function, integrity, and hemodynamic parameters are evaluated for each group.

Results: The present experimental data show that pretreatment with IrA reduced the LDH, CK-MB, size of myocardial infarction, content of cardiac collagen, and ventricular fibrillation in all groups of HL rat hearts. This pretreatment also reduced the oxidative stress and mitochondrial dysfunction. Inhibition of MPTP opening by Atr diminished the effect of IrA on IPC-mediated cardioprotection in HL rats.

Conclusion: The study findings indicate that pretreatment with IrA e restores IPC-mediated cardioprotection in HL rats by inhibiting the MPTP opening.

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总状菊提取物对高脂血症大鼠缺血预处理心脏保护作用的调节作用。

目的:高脂血症(HL)是缺血性心脏病的主要病因。缺血预处理(IPC)的大小限制作用，一种心脏保护现象，在HL中减少，可能是由于线粒体通透性过渡孔(MPTP)的开放。本研究旨在观察总状菊钩根提取物(IrA)预处理对ipc介导的HL Wistar大鼠心脏保护作用的影响。将离体大鼠心脏置于Langendorff心脏阵列上，进行缺血再灌注(I/R)和IPC循环。苍术苷(Atr)是MPTP开启器。方法:将大鼠分为10组，每组6只(n = 6)，观察总形藤提取物对HL心脏IPC保护作用的调节作用，分别为假对照组、I/R对照组、IPC对照组、I/R + HL、I/R + IrA+ HL、IPC + HL、IPC + NS + HL、IPC + IrA+ HL、IPC + Atr +氧化应激，评价各组线粒体功能、完整性和血流动力学参数。结果:本实验数据显示，IrA预处理可降低各组HL大鼠心脏LDH、CK-MB、心肌梗死大小、心肌胶原含量及心室颤动。预处理还可降低氧化应激和线粒体功能障碍。Atr对MPTP开放的抑制降低了IrA对HL大鼠ipc介导的心脏保护作用。结论:研究结果表明，IrA e预处理可通过抑制MPTP开放来恢复ipc介导的HL大鼠心脏保护作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Pharmacopuncture INTEGRATIVE & COMPLEMENTARY MEDICINE-

CiteScore

2.10

自引率

7.10%

发文量

审稿时长

10 weeks

期刊介绍： The Journal of Pharmacopuncture covers a wide range of basic and clinical science research relevant to all aspects of the biotechnology of integrated approaches using both pharmacology and acupuncture therapeutics, including research involving pharmacology, acupuncture studies and pharmacopuncture studies. The subjects are mainly divided into three categories: pharmacology (applied phytomedicine, plant sciences, pharmacology, toxicology, medicinal plants, traditional medicines, herbal medicine, Sasang constitutional medicine, herbal formulae, foods, agricultural technologies, naturopathy, etc.), acupuncture (acupressure, electroacupuncture, laser acupuncture, moxibustion, cupping, etc.), and pharmacopuncture (aqua-acupuncture, meridian pharmacopuncture, eight-principles pharmacopuncture, animal-based pharmacopuncture, mountain ginseng pharmacopuncture, bee venom therapy, needle embedding therapy, implant therapy, etc.). Other categories include chuna treatment, veterinary acupuncture and related animal studies, alternative medicines for treating cancer and cancer-related symptoms, etc. Broader topical coverage on the effects of acupuncture, the medical plants used in traditional and alternative medicine, pharmacological action and other related modalities, such as anthroposophy, homeopathy, ayurveda, bioelectromagnetic therapy, chiropractic, neural therapy and meditation, can be considered to be within the journal’s scope if based on acupoints and meridians. Submissions of original articles, review articles, systematic reviews, case reports, brief reports, opinions, commentaries, medical lectures, letters to the editor, photo-essays, technical notes, and book reviews are encouraged. Providing free access to the full text of all current and archived articles on its website (www.journal.ac), also searchable through a Google Scholar search.