Biodegradasi Pewarna Tekstil Rhodamin B oleh Bakteri Pembentuk Biofilm

Nurlela Yanti, Sitti Wirdhana Ahmad, Fatimah Alwi Albakar
{"title":"Biodegradasi Pewarna Tekstil Rhodamin B oleh Bakteri Pembentuk Biofilm","authors":"Nurlela Yanti, Sitti Wirdhana Ahmad, Fatimah Alwi Albakar","doi":"10.20961/ALCHEMY.17.2.49010.151-158","DOIUrl":null,"url":null,"abstract":"<p id=\"docs-internal-guid-91ba8b82-7fff-b26e-92e4-41a17a9eec57\" style=\"line-height: 1.2; margin-left: 0.35pt; margin-right: -0.1pt; text-align: justify; margin-top: 6pt; margin-bottom: 6pt;\" dir=\"ltr\"><span style=\"font-size: 8.5pt; font-family: 'Times New Roman'; color: #000000; background-color: transparent; font-weight: 400; font-style: normal; font-variant: normal; text-decoration: none; vertical-align: baseline; white-space: pre-wrap;\">Rhodamin B merupakan salah satu pewarna yang paling sering digunakan pada industri tekstil. Limbah pewarna tekstil ini dapat mencemari lingkungan jika masuk ke lingkungan melebihi ambang batas normal. Penggunaan bakteri pembentuk biofilm sebagai agensia pendegradasi Rhodamin B sangat memungkinkan karena mampu meningkatkan degradasi senyawa melalui interaksi antar bakteri sehingga mampu melengkapi proses metabolik. Tujuan penelitian ini adalah untuk mengetahui kemampuan isolat bakteri lokal pembentuk biofilm dalam mendegradasi pewarna tekstil Rhodamin B. Penelitian ini menggunakan 3 isolat bakteri pembentuk biofilm yang diperoleh dari perairan Tanjung Tiram, Kabupaten Konawe Selatan, Sulawesi Tenggara, yaitu isolat bakteri </span><span style=\"font-size: 8.5pt; font-family: 'Times New Roman'; color: #000000; background-color: transparent; font-weight: 400; font-style: italic; font-variant: normal; text-decoration: none; vertical-align: baseline; white-space: pre-wrap;\">Chromohalobacter</span><span style=\"font-size: 8.5pt; font-family: 'Times New Roman'; color: #000000; background-color: transparent; font-weight: 400; font-style: italic; font-variant: normal; text-decoration: none; vertical-align: baseline; white-space: pre-wrap;\">sp.</span><span style=\"font-size: 8.5pt; font-family: 'Times New Roman'; color: #000000; background-color: transparent; font-weight: 400; font-style: normal; font-variant: normal; text-decoration: none; vertical-align: baseline; white-space: pre-wrap;\"> BfTT2.1.5, </span><span style=\"font-size: 8.5pt; font-family: 'Times New Roman'; color: #000000; background-color: transparent; font-weight: 400; font-style: italic; font-variant: normal; text-decoration: none; vertical-align: baseline; white-space: pre-wrap;\">Bacillus</span><span style=\"font-size: 8.5pt; font-family: 'Times New Roman'; color: #000000; background-color: transparent; font-weight: 400; font-style: italic; font-variant: normal; text-decoration: none; vertical-align: baseline; white-space: pre-wrap;\">sp. </span><span style=\"font-size: 8.5pt; font-family: 'Times New Roman'; color: #000000; background-color: transparent; font-weight: 400; font-style: normal; font-variant: normal; text-decoration: none; vertical-align: baseline; white-space: pre-wrap;\">BfTT2.11 dan </span><span style=\"font-size: 8.5pt; font-family: 'Times New Roman'; color: #000000; background-color: transparent; font-weight: 400; font-style: italic; font-variant: normal; text-decoration: none; vertical-align: baseline; white-space: pre-wrap;\">Vibrio</span><span style=\"font-size: 8.5pt; font-family: 'Times New Roman'; color: #000000; background-color: transparent; font-weight: 400; font-style: italic; font-variant: normal; text-decoration: none; vertical-align: baseline; white-space: pre-wrap;\">sp.</span><span style=\"font-size: 8.5pt; font-family: 'Times New Roman'; color: #000000; background-color: transparent; font-weight: 400; font-style: normal; font-variant: normal; text-decoration: none; vertical-align: baseline; white-space: pre-wrap;\"> BfTT2.14. Kemampuan biodegradasi Rhodamin B oleh isolat bakteri pembentuk biofilm dideteksi menggunakan tiga parameter yaitu dekolorisasi pewarna Rhodamin B menggunakan metode spektrofotometri, kadar CO</span><span style=\"font-size: 8.5pt; font-family: 'Times New Roman'; color: #000000; background-color: transparent; font-weight: 400; font-style: normal; font-variant: normal; text-decoration: none; vertical-align: baseline; white-space: pre-wrap;\"><span style=\"font-size: 0.6em; vertical-align: sub;\">2</span></span><span style=\"font-size: 8.5pt; font-family: 'Times New Roman'; color: #000000; background-color: transparent; font-weight: 400; font-style: normal; font-variant: normal; text-decoration: none; vertical-align: baseline; white-space: pre-wrap;\"> terlarut menggunakan metode titrasi dan total sel bakteri menggunakan metode </span><span style=\"font-size: 8.5pt; font-family: 'Times New Roman'; color: #000000; background-color: transparent; font-weight: 400; font-style: italic; font-variant: normal; text-decoration: none; vertical-align: baseline; white-space: pre-wrap;\">drop plate</span><span style=\"font-size: 8.5pt; font-family: 'Times New Roman'; color: #000000; background-color: transparent; font-weight: 400; font-style: normal; font-variant: normal; text-decoration: none; vertical-align: baseline; white-space: pre-wrap;\">. Berdasarkan uji kemampuan dalam mendegradasi pewarna tekstil Rhodamin B, </span><span style=\"font-size: 8.5pt; font-family: 'Times New Roman'; color: #000000; background-color: transparent; font-weight: 400; font-style: italic; font-variant: normal; text-decoration: none; vertical-align: baseline; white-space: pre-wrap;\">Chromohalobacter sp.</span><span style=\"font-size: 8.5pt; font-family: 'Times New Roman'; color: #000000; background-color: transparent; font-weight: 400; font-style: normal; font-variant: normal; text-decoration: none; vertical-align: baseline; white-space: pre-wrap;\"> BfTT2.1.5 mampu mendegradasi Rhodamin B sebanyak 36,1% lebih tinggi dibanding </span><span style=\"font-size: 8.5pt; font-family: 'Times New Roman'; color: #000000; background-color: transparent; font-weight: 400; font-style: italic; font-variant: normal; text-decoration: none; vertical-align: baseline; white-space: pre-wrap;\">Bacillus sp.</span><span style=\"font-size: 8.5pt; font-family: 'Times New Roman'; color: #000000; background-color: transparent; font-weight: 400; font-style: normal; font-variant: normal; text-decoration: none; vertical-align: baseline; white-space: pre-wrap;\"> BfTT2.11 (33,24%) dan </span><span style=\"font-size: 8.5pt; font-family: 'Times New Roman'; color: #000000; background-color: transparent; font-weight: 400; font-style: italic; font-variant: normal; text-decoration: none; vertical-align: baseline; white-space: pre-wrap;\">Vibrio</span><span style=\"font-size: 8.5pt; font-family: 'Times New Roman'; color: #000000; background-color: transparent; font-weight: 400; font-style: italic; font-variant: normal; text-decoration: none; vertical-align: baseline; white-space: pre-wrap;\">sp.</span><span style=\"font-size: 8.5pt; font-family: 'Times New Roman'; color: #000000; background-color: transparent; font-weight: 400; font-style: normal; font-variant: normal; text-decoration: none; vertical-align: baseline; white-space: pre-wrap;\"> BfTT2.14 (16,18%). Ketiga isolat bakteri pembentuk biofilm mampu menggunakan Rhodamin B sebagai sumber karbon untuk pertumbuhannya. Oleh karena itu, isolat bakteri pembentuk biofilm sangat berpotensi digunakan sebagai agensia bioremediasi pewarna tekstil Rhodamin B.</span></p><p id=\"docs-internal-guid-148234b8-7fff-db03-2081-70b339674f74\" style=\"line-height: 1.2; margin-left: 0.35pt; margin-right: -0.1pt; text-align: justify; margin-top: 6pt; margin-bottom: 6pt;\" dir=\"ltr\"><strong><span style=\"font-size: 8.5pt; font-family: 'Times New Roman'; color: #000000; background-color: transparent; font-style: normal; font-variant: normal; text-decoration: none; vertical-align: baseline; white-space: pre-wrap;\">Biodegradation of Textile Dye Rhodamine B Using Biofilm-forming Bacteria. </span></strong><span style=\"font-size: 8.5pt; font-family: 'Times New Roman'; color: #000000; background-color: transparent; font-weight: 400; font-style: normal; font-variant: normal; text-decoration: none; vertical-align: baseline; white-space: pre-wrap;\">Rhodamine B is one of the most widely used dyes in the textile industry.</span><span style=\"font-size: 8.5pt; font-family: 'Times New Roman'; color: #000000; background-color: transparent; font-weight: 400; font-style: normal; font-variant: normal; text-decoration: none; vertical-align: baseline; white-space: pre-wrap;\"> This textile dye waste can pollute the environment if it enters the environment beyond the normal threshold. The use of biofilm-forming bacteria as agents to degrade Rhodamine B is possible because it can increase the degradation of compounds through interactions between bacteria to complete the metabolic process. The purpose of this study was to find out the resistance of local biofilm-forming bacteria isolates to Rhodamine B textile dyes and to find out the ability of local biofilm-forming bacterial isolates in degrading Rhodamine B textile dyes. This study used three isolates of biofilm-producing bacteria obtained from the waters of Tanjung Tiram, Konawe Selatan Regency, Southeast Sulawesi, namely </span><span style=\"font-size: 8.5pt; font-family: 'Times New Roman'; color: #000000; background-color: transparent; font-weight: 400; font-style: italic; font-variant: normal; text-decoration: none; vertical-align: baseline; white-space: pre-wrap;\">Chromohalobacter</span><span style=\"font-size: 8.5pt; font-family: 'Times New Roman'; color: #000000; background-color: transparent; font-weight: 400; font-style: italic; font-variant: normal; text-decoration: none; vertical-align: baseline; white-space: pre-wrap;\">sp. </span><span style=\"font-size: 8.5pt; font-family: 'Times New Roman'; color: #000000; background-color: transparent; font-weight: 400; font-style: normal; font-variant: normal; text-decoration: none; vertical-align: baseline; white-space: pre-wrap;\">BfTT2.1.5, </span><span style=\"font-size: 8.5pt; font-family: 'Times New Roman'; color: #000000; background-color: transparent; font-weight: 400; font-style: italic; font-variant: normal; text-decoration: none; vertical-align: baseline; white-space: pre-wrap;\">Bacillus</span><span style=\"font-size: 8.5pt; font-family: 'Times New Roman'; co","PeriodicalId":7926,"journal":{"name":"Alchemy: Jurnal Penelitian Kimia","volume":"71 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2021-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Alchemy: Jurnal Penelitian Kimia","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.20961/ALCHEMY.17.2.49010.151-158","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
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Abstract

Rhodamin B merupakan salah satu pewarna yang paling sering digunakan pada industri tekstil. Limbah pewarna tekstil ini dapat mencemari lingkungan jika masuk ke lingkungan melebihi ambang batas normal. Penggunaan bakteri pembentuk biofilm sebagai agensia pendegradasi Rhodamin B sangat memungkinkan karena mampu meningkatkan degradasi senyawa melalui interaksi antar bakteri sehingga mampu melengkapi proses metabolik. Tujuan penelitian ini adalah untuk mengetahui kemampuan isolat bakteri lokal pembentuk biofilm dalam mendegradasi pewarna tekstil Rhodamin B. Penelitian ini menggunakan 3 isolat bakteri pembentuk biofilm yang diperoleh dari perairan Tanjung Tiram, Kabupaten Konawe Selatan, Sulawesi Tenggara, yaitu isolat bakteri Chromohalobactersp. BfTT2.1.5, Bacillussp. BfTT2.11 dan Vibriosp. BfTT2.14. Kemampuan biodegradasi Rhodamin B oleh isolat bakteri pembentuk biofilm dideteksi menggunakan tiga parameter yaitu dekolorisasi pewarna Rhodamin B menggunakan metode spektrofotometri, kadar CO2 terlarut menggunakan metode titrasi dan total sel bakteri menggunakan metode drop plate. Berdasarkan uji kemampuan dalam mendegradasi pewarna tekstil Rhodamin B, Chromohalobacter sp. BfTT2.1.5 mampu mendegradasi Rhodamin B sebanyak 36,1% lebih tinggi dibanding Bacillus sp. BfTT2.11 (33,24%) dan Vibriosp. BfTT2.14 (16,18%). Ketiga isolat bakteri pembentuk biofilm mampu menggunakan Rhodamin B sebagai sumber karbon untuk pertumbuhannya. Oleh karena itu, isolat bakteri pembentuk biofilm sangat berpotensi digunakan sebagai agensia bioremediasi pewarna tekstil Rhodamin B.

Biodegradation of Textile Dye Rhodamine B Using Biofilm-forming Bacteria. Rhodamine B is one of the most widely used dyes in the textile industry. This textile dye waste can pollute the environment if it enters the environment beyond the normal threshold. The use of biofilm-forming bacteria as agents to degrade Rhodamine B is possible because it can increase the degradation of compounds through interactions between bacteria to complete the metabolic process. The purpose of this study was to find out the resistance of local biofilm-forming bacteria isolates to Rhodamine B textile dyes and to find out the ability of local biofilm-forming bacterial isolates in degrading Rhodamine B textile dyes. This study used three isolates of biofilm-producing bacteria obtained from the waters of Tanjung Tiram, Konawe Selatan Regency, Southeast Sulawesi, namely Chromohalobactersp. BfTT2.1.5, Bacillus

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生物降解染料罗达敏B由制造生物薄膜的细菌制成
Rhodamin B是纺织业最常用的染料之一。如果环境超出正常范围,这些纺织品废物就会污染环境。将生物胶片分层的细菌作为增殖体B有可能通过细菌之间的相互作用促进化合物的退化,从而完成代谢过程。本研究的目的是确定一种当地的细菌是如何在tridamin B染色方面进行生物测定的。该研究使用了从南苏拉威西东南部角清普亭(conawe sublicament)的三种分离型生物胶片。1。5,Bacillussp BfTT2。BfTT2.11和弧菌。BfTT2 14。利用光谱分析法,利用着色方法,利用滴落法,利用细菌的总细胞,发现了合成微生物的生物降解特性。根据检测检测检测检测检测检测检测检测检测检测检测检测检测检测检测检测检测检测检测结果BfTT2 14(16,18%)。仿生学的三种分离细菌能够利用小红素B作为生长的碳来源。因此,仿生学的细菌分离是一种极具潜力的染料,用于草本植物色素制毒。Rhodamine B是纺织行业中最聪明的人之一。如果把环境与正常环境之外的环境结合起来,这种布料就会污染环境。用生物胶片使细菌退化成为可能,因为它可以通过细菌之间的相互作用增加化合物的退化来完成代谢过程。这项研究的目的是发现局部生物生成的异质细菌对路胺B textile dyes,并在degramine B textile dyes中发现局部生物生成异化细菌的能力。这项研究利用了三种来自好望角、苏里东、苏拉威西东南部、纳梅尔伊·克莫莫内格罗特斯的生物生成细菌的孤立。bftt2.1.5, Bacillus
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