A methodology to evaluate different histological preparations of soft tissues: Intervertebral disc tissues study.

IF 3.1 4区 医学 Q2 BIOPHYSICS
Azril, Kuo-Yuan Huang, Jonathan Hobley, Mehdi Rouhani, Wen-Lung Liu, Yeau-Ren Jeng
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引用次数: 0

Abstract

A tissue preparation method will inevitably alter the tissue content. This study aims to evaluate how different common sample preparation methods will affect the tissue morphology, biomechanical properties, and chemical composition of samples. The study focuses on intervertebral disc (IVD) tissue; however, it can be applied to other soft tissues. Raman spectroscopy synchronized with nanoindentation instrumentation was employed to investigate the compositional changes of IVD, specifically, nucleus pulposus (NP) and annulus fibrosus (AF), together with their biomechanical properties of IVD. These properties were examined through the following histological specimen types: fresh cryosection (control), fixed cryosection, and paraffin-embedded. The IVD tissue could be located using an optical microscope under three different preparation methods. Paraffin-embedded samples showed the most explicit details where the lamellae structure of AF could be identified. In terms of biomechanical properties, there was no significant difference between the fresh and fixed cryosection (p > 0.05). In contrast, the fresh cryosection and paraffin-embedded samples showed a significant difference (p < 0.05). It was also found that the tissue preparations affected the chemical content of the tissues and structure of the tissue, which are expected to contribute to biomechanical properties changes. Fresh cryosection and fixed cryosection samples are more promising to work with for biomechanical assessment in histological tissues. The findings fill essential gaps in the literature by providing valuable insight into the characteristics of IVD at the microscale. This study can also become a reference for a better approach to assessing the mechanical properties and chemical content of soft tissues at the microscale.

评估不同软组织组织学准备的方法:椎间盘组织研究。
组织制备方法不可避免地会改变组织含量。本研究旨在评估不同的常见样品制备方法对样品的组织形态、生物力学性能和化学成分的影响。该研究的重点是椎间盘(IVD)组织;然而,它可以应用于其他软组织。采用拉曼光谱同步纳米压痕仪研究了IVD的组成变化,即髓核(NP)和纤维环(AF),以及它们的生物力学特性。这些特性通过以下组织学标本类型进行检测:新鲜冷冻切片(对照),固定冷冻切片和石蜡包埋。在三种不同的制备方法下,使用光学显微镜可以定位IVD组织。石蜡包埋的样品显示了AF的片层结构最清晰的细节。在生物力学性能方面,新鲜冷冻切片与固定冷冻切片的差异无统计学意义(p > 0.05)。相比之下,新鲜冷冻切片和石蜡包埋样品显示出显著差异(p
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来源期刊
Journal of Applied Biomaterials & Functional Materials
Journal of Applied Biomaterials & Functional Materials BIOPHYSICS-ENGINEERING, BIOMEDICAL
CiteScore
4.40
自引率
4.00%
发文量
36
审稿时长
>12 weeks
期刊介绍: The Journal of Applied Biomaterials & Functional Materials (JABFM) is an open access, peer-reviewed, international journal considering the publication of original contributions, reviews and editorials dealing with clinical and laboratory investigations in the fast growing field of biomaterial sciences and functional materials. The areas covered by the journal will include: • Biomaterials / Materials for biomedical applications • Functional materials • Hybrid and composite materials • Soft materials • Hydrogels • Nanomaterials • Gene delivery • Nonodevices • Metamaterials • Active coatings • Surface functionalization • Tissue engineering • Cell delivery/cell encapsulation systems • 3D printing materials • Material characterization • Biomechanics
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