RIPENING DELAY OF ‘SABA’ BANANA USING CHITOSAN COATING MATERIAL FROM THE TEST WASTES OF PHILIPPINE BLUE-EYED SEA URCHIN

Betti Mae C. Mendoza, Neil Ian V. Magalang, Aurora Blanca M. Lalo, R. M. Ureta
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Abstract

Postharvest loss of bananas due to ripening acceleration is one of the problems of local Filipino farmers. This problem has resulted in profit losses which could bring serious effects in the agricultural sector. Thus, this study aims to derive chitosan, a biopolymer and non-toxic material from the test wastes of the Philippine blue-eyed sea urchins (Diadema savignyi Michelin, 1845) to be used as a surface coating for ‘Saba’ banana (Musa x paradisiaca) to delay the ripening process. The chitosan was obtained by decolorization, pulverization, deproteinization, demineralization, and deacetylation of the sea urchins’ tests. The derived chitosan was validated by its Degree of Acetylation (DA) (35.65%) based on amino and hydroxyl group at peaks 1645.25 cm-1 and 3469.63 cm-1. Scanning Electron Microscopy (SEM) micrographs confirmed the amorphous and non-homogeneous structure of chitosan. Results also showed that the chitosan-coated bananas had lower weight loss, greener color,  firmer, and lower disease incidence after the seven-day observation as compared with that of the uncoated samples. The study implies that the synthesized chitosan from the test wastes of Philippine blue-eyed sea urchin can be a potential natural coating material in delaying the rapid ripening of ‘Saba’ banana.
菲律宾蓝眼海胆试验废弃物壳聚糖包衣材料延缓“沙巴”香蕉成熟
香蕉因成熟加速而导致的采后损失是菲律宾当地农民面临的问题之一。这一问题造成了利润损失,可能给农业部门带来严重影响。因此,本研究旨在从菲律宾蓝眼海胆(Diadema savignyi Michelin, 1845)的试验废物中提取生物聚合物和无毒材料壳聚糖,用于“Saba”香蕉(Musa x paradisiaca)的表面涂层,以延缓成熟过程。对海胆样品进行脱色、粉碎、脱蛋白、脱矿、脱乙酰等处理,得到壳聚糖。壳聚糖在1645.25 cm-1和3469.63 cm-1峰上的氨基和羟基的乙酰化度(DA)为35.65%。扫描电子显微镜(SEM)证实了壳聚糖的无定形和非均匀结构。结果还表明,经过7天的观察,壳聚糖包被的香蕉与未包被的香蕉相比,重量减轻,颜色更绿,更结实,发病率更低。该研究表明,从菲律宾蓝眼海胆试验废料中合成的壳聚糖可以作为一种潜在的天然涂层材料,延缓“萨巴”香蕉的快速成熟。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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