Effects of second-generation H1-antihistamine drugs on angiogenesis in in vivo chick chorioallantoic membrane model.

IF 1.6 4区 医学 Q3 OPHTHALMOLOGY
Nilay Duman, Reşat Duman, Ayhan Vurmaz
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引用次数: 0

Abstract

Background: Literature on the effects of second-generation H1-antihistamines on angiogenesis is limited.

Objectives: To investigate the effects of cetirizine, desloratadine, and rupatadine (second-generation H1-antihistamines commonly used in dermatology clinics) on angiogenesis in an in vivo chick chorioallantoic membrane (CAM) model.

Methods: The study was approved by the local ethics committee on animal experimentation. Forty fertilized specific pathogen free eggs were incubated and kept under appropriate temperature and humidity control. Drug solutions were prepared in identical concentrations by dissolving powders in phosphate-buffered saline (PBS). On the third day of the incubation, a small window was opened on the CAM and 0.1 mL desloratadine (1.5 μg/0.1 mL) in the first group, 0.1 mL cetirizine (1.5 μg/0.1 mL) in the second group, 0.1 mL rupatadine in the third group (1.5 μg/0.1 mL), and PBS (0.1 mL) in the fourth group were administered by injection. On the eighth day of incubation, the vascular structures of the CAMs were macroscopically examined and standard digital photographs were taken. The digital images were analyzed and data including mean vessel density, thickness, and number were compared between groups. p < 0.05 was considered statistically significant.

Results: Vessel densities were similar in the desloratadine, cetirizine, and control groups, whereas they were significantly less in the rupatadine group (p = 0.01). Furthermore, the rupatadine group had significantly lower vessel thickness and number compared with the other groups (p < 0.05 for both).

Conclusions: Rupatadine showed anti-angiogenic effects in the chick CAM model, compared with desloratadine and cetirizine. The anti-angiogenic effect of rupatadine could be due to its platelet-activating factor (PAF) receptor inhibition. Thus, rupatadine could be a treatment agent in pathological processes in which angiogenesis is responsible. Further studies with larger series are needed to clarify this potential.

第二代h1 -抗组胺药对鸡绒毛膜尿囊膜模型血管生成的影响。
背景:关于第二代h1 -抗组胺药对血管生成影响的文献有限。目的:探讨西替利嗪、地氯雷他定和鲁帕他定(皮肤科临床常用的第二代抗组胺药)对鸡绒毛膜尿囊膜(CAM)模型血管生成的影响。方法:本研究经当地动物实验伦理委员会批准。在适当的温度和湿度下孵育40个特定的无致病菌受精卵。将粉末溶解在磷酸盐缓冲盐水(PBS)中,以相同浓度制备药物溶液。孵育第3天,在CAM上开小窗,第一组注射0.1 mL地氯雷他定(1.5 μg/0.1 mL),第二组注射0.1 mL西替利嗪(1.5 μg/0.1 mL),第三组注射0.1 mL鲁帕他定(1.5 μg/0.1 mL),第四组注射0.1 mL PBS (0.1 mL)。在培养的第8天,对cam的血管结构进行宏观检查,并拍摄标准的数码照片。对数字图像进行分析,并比较两组间平均血管密度、厚度、数量等数据。结果:地氯雷他定组、西替利嗪组和对照组血管密度相似,鲁帕他定组血管密度明显降低(p = 0.01)。结论:与地氯雷他定和西替利嗪相比,鲁帕他定在鸡CAM模型中具有抗血管生成作用。鲁帕他定的抗血管生成作用可能是由于其抑制血小板活化因子(PAF)受体。因此,鲁帕他定可能是一种治疗血管生成的病理过程的药物。需要更大规模的进一步研究来阐明这种可能性。
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来源期刊
CiteScore
3.30
自引率
6.20%
发文量
40
审稿时长
1 months
期刊介绍: Cutaneous and Ocular Toxicology is an international, peer-reviewed journal that covers all types of harm to cutaneous and ocular systems. Areas of particular interest include pharmaceutical and medical products; consumer, personal care, and household products; and issues in environmental and occupational exposures. In addition to original research papers, reviews and short communications are invited, as well as concise, relevant, and critical reviews of topics of contemporary significance.
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