Evaluation of selective laser sintered polyamide/hydroxyapatite composite compositions in vitro and in vivo

International journal of biomedical research Pub Date : 2017-08-30 DOI:10.7439/IJBR.V8I8.4322

Banu Pradheepa Kamarajan, R. Murugan, Ananthasubramanian Muthusamy, K. DinakarRaiB, Vignesh Mathialagan, S. Shanthakumari

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引用次数: 2

Abstract

Objective: To study the suitability of the developed polyamide/hydroxyapatite (PA/HA) composite orthopaedic scaffold in vitro and in vivo. Methods: The fabricated scaffolds were seeded with human osteoblast-like cell line (MG 63) and tested for its viability & cytotoxicity, proliferation and calcium mineralization through MTT assay, ALP assay and Alizarin red S staining respectively. Finally, the scaffolds were implanted in non-immunocompromised male wistar rats subcutaneously and pathological tests were carried out. Results: The results of in vitro studies indicate that PA/HA (90:10) composite was non-cytotoxic and supported calcium mineralization relatively better than the other compositions of PA/HA. From the Hematoxylin & Eosin staining, inflammation was observed in the tissues surrounding the scaffold 7 weeks post implantation in rats. However, the scaffolds were found to be incorporated into the subcutaneous tissue at the end of one year without any rejection. Conclusion: PA/HA (90:10) composite scaffold fabricated using SLS is biocompatible when tested in rats, and could more likely be used for orthopedic applications.

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选择性激光烧结聚酰胺/羟基磷灰石复合材料的体外和体内评价

目的:研究聚酰胺/羟基磷灰石(PA/HA)复合骨科支架在体外和体内的适用性。方法:用人成骨样细胞系mg63植入支架，分别采用MTT法、ALP法和茜素红S染色检测支架的细胞活力、细胞毒性、增殖能力和钙矿化程度。最后，将支架植入非免疫功能低下的雄性wistar大鼠皮下并进行病理检查。结果:体外实验结果表明，PA/HA(90:10)复合物比其他PA/HA复合物具有较好的细胞毒性和支持钙矿化作用。苏木精和伊红染色显示大鼠支架周围组织在植入7周后出现炎症反应。然而，发现支架在一年后被纳入皮下组织，没有任何排斥反应。结论:SLS制备的PA/HA(90:10)复合支架在大鼠体内具有良好的生物相容性，更有可能用于骨科。

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International journal of biomedical research

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