COFRADIC™: the Hubble telescope of proteomics

Abstract

COFRADIC™ is a diagonal chromatographic technique designed to select peptides from complex mixtures. It consists of two consecutive identical chromatographic separations, with a modification step targeted to a subset of peptides between the two separations. The modified peptides obtain different chromatographic properties and segregate from the bulk of unaltered peptides in the second run. The final analysis is limited to the sorted peptides, thereby reducing the complexity but keeping all of the characteristics of the proteome.