Effects of different cytokinins on in vitro multiplication of Prunus 'Capdeboscq' rootstocks

IF 1.3 4区农林科学 Q3 AGRONOMY

Crop Breeding and Applied Biotechnology Pub Date : 2003-06-30 DOI:10.12702/1984-7033.V03N02A08

Aparecido Lima da Silva, M. Rogalski, M. P. Guerra

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引用次数: 11

Abstract

Rootstocks for peach trees are obtained in Brazil from seeds without genetic uniformity or health guarantee. Micropropagation techniques supply genetically uniform shoots but the in vitro culture protocols must be optimized. Cytokinins are essential for breaking the apex dormancy and inducing auxiliary shoot proliferation that influence the success of in vitro multiplication. The objective of this study was to assess the effect of different cytokinins in vitro multiplication rate, and on the survival rate of apical and axyllary buds in the establishment of Prunus ‘Capdeboscq’ rootstocks. Apical and axyllary buds were inoculated in test tubes containing 10mL Lepoivre culture medium, supplemented with BAP (0.5 mg L -1 ). After two subcultures of the in vitro establishment process, 1-2 cm nodal segments were treated with kinetin, isopentenyladenine and BAP, at concentrations of 0.1; 0.5; 1.0 and 2.0 mg L -1 . At the in vitro establishment phase, stem tips presented greater survival, less contamination and no hyperhidricity. BAP gave the highest in vitro multiplication rate, with 25.9 shoots/explant at the 1.5 mg L -1 . The different cytokinins did not result in differences in shoot height. The best results for the number of shoots greater than 20 mm were obtained with BAP at 1.0 mg L -1 .

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不同细胞分裂素对山李砧木离体增殖的影响

巴西桃树的砧木是由没有遗传一致性或健康保证的种子获得的。微繁技术提供遗传上均匀的芽，但离体培养方案必须优化。细胞分裂素是打破茎尖休眠和诱导辅助芽增殖的重要物质，影响离体增殖的成功与否。本研究旨在探讨不同细胞分裂素对山李砧木培养过程中根尖芽和腋芽成活率的影响。顶芽和腋芽接种于含有10mL Lepoivre培养基的试管中，并添加BAP (0.5 mg L -1)。体外培养2次后，分别用0.1浓度的动蛋白、异戊烯腺嘌呤和BAP处理1-2 cm的节段;0.5;1.0和2.0 mg L -1。在离体培养阶段，茎尖表现出更高的存活率，更少的污染和无多汗症。在1.5 mg L -1浓度下，BAP的体外增殖率最高，为25.9个芽/外植体。不同的细胞分裂素对茎高没有影响。BAP浓度为1.0 mg L -1时，芽数大于20 mm的效果最好。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Crop Breeding and Applied Biotechnology AGRONOMY-BIOTECHNOLOGY & APPLIED MICROBIOLOGY

CiteScore

2.70

自引率

13.30%

发文量

审稿时长

6-12 weeks

期刊介绍： The CBAB – CROP BREEDING AND APPLIED BIOTECHNOLOGY (ISSN 1984-7033) – is the official quarterly journal of the Brazilian Society of Plant Breeding, abbreviated CROP BREED APPL BIOTECHNOL. It publishes original scientific articles, which contribute to the scientific and technological development of plant breeding and agriculture. Articles should be to do with basic and applied research on improvement of perennial and annual plants, within the fields of genetics, conservation of germplasm, biotechnology, genomics, cytogenetics, experimental statistics, seeds, food quality, biotic and abiotic stress, and correlated areas. The article must be unpublished. Simultaneous submitting to another periodical is ruled out. Authors are held solely responsible for the opinions and ideas expressed, which do not necessarily reflect the view of the Editorial board. However, the Editorial board reserves the right to suggest or ask for any modifications required. The journal adopts the Ithenticate software for identification of plagiarism. Complete or partial reproduction of articles is permitted, provided the source is cited. All content of the journal, except where identified, is licensed under a Creative Commons attribution-type BY. All articles are published free of charge. This is an open access journal.