Determination of Optimum Immobilization Conditions of Trametes versicolor Laccase with Sodium Alginate Beads

Abstract

Laccase ( EC 1.10.3.2) is a multi copper enzyme that catalyzes the oxidation of various environmental pollutants such as phenolic compounds. The efficiency of the enzyme for environmental and industrial applications can be increased by immobilizing the enzyme on a carrier. In the present study, laccase obtained from Trametes versicolor (fungi) was immobilized on sodium alginate beads and kappacarrageenan and the effect of contact time, pH, temperature, the amount of carrier and enzyme concentration were investigated to determine optimum conditions of laccase immobilization. Sodium alginate beads were chosen as the most efficient carrier due to their high immobilization yield. Maximum laccase immobilization was determined as 30 min of contact time, pH 4.5, 30 °C of temperature, 200 mg of sodium alginate amount.