Antioxidant activity and protective effects of commercial propolis on gentamicin induced nephrotoxicity in rabbits- In vitro study

IF 0.6 4区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Turkish Journal of Biochemistry-turk Biyokimya Dergisi Pub Date : 2013-01-01 DOI:10.5505/TJB.2013.29200

I. H. Osman, Ahmed Abdel Hafez Tantaway

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引用次数: 6

Abstract

Objective: the aim of this study is to investigate the in vitro antioxidant activity, the total phenol and flavonoid content and the possible protective effects of commercial propolis on gentamicin-induced nephrotoxicity in rabbits. Methods: the in vitro antioxidant activity was measured by ferric reducing antioxidant power assay and cupric reducing antioxidant capacity assay, the total phenols content was measured by folin–ciocalteau assay, the flavonoids content by the alcl3 colorimetric method and the renoprotective effects of propolis methanol extract was evaluated in a rabbit model of gentamicin-induced nephrotoxicity. The protective effects of propolis on gentamicin-induced nephrotoxicity in rabbits were evaluated through biochemical parameter (measuring serum urea and creatinine) and histopathological alterations in kidneys Results: methanol extract of propolis showed a strong antioxidant activity, which is attributed to its high phenolic and flavonoid contents. Oral administration of propolis extract to rabbits at a dose of 1 mg/kg body weight significantly protected against histopathological and biochemical alterations induced by gentamicin. Conclusion: the present study demonstrated that commercial propolis is strong antioxidant and is effective for the prevention of gentamicin-induced renal damage in rabbits

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商用蜂胶对庆大霉素致兔肾毒性的抗氧化活性和保护作用的体外研究

目的:研究商业蜂胶的体外抗氧化活性、总酚和总黄酮含量以及对庆大霉素致家兔肾毒性的保护作用。方法:采用铁还原抗氧化能力法和铜还原抗氧化能力法测定蜂胶的体外抗氧化活性，用folin-ciocalteau法测定蜂胶中总酚含量，用alcl3比色法测定蜂胶中总黄酮含量，并采用庆大霉素肾毒性家兔模型评价蜂胶甲醇提取物的肾保护作用。结果:蜂胶甲醇提取物具有较强的抗氧化活性，这与蜂胶中酚类和类黄酮含量较高有关。以1 mg/kg体重剂量口服蜂胶提取物对庆大霉素引起的组织病理和生化改变有显著的保护作用。结论:商用蜂胶具有较强的抗氧化作用，对庆大霉素致家兔肾损伤有较好的预防作用

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来源期刊

Turkish Journal of Biochemistry-turk Biyokimya Dergisi 生物-生化与分子生物学

CiteScore

1.20

自引率

0.00%

发文量

审稿时长

6-12 weeks

期刊介绍： Turkish Journal of Biochemistry (TJB), official journal of Turkish Biochemical Society, is issued electronically every 2 months. The main aim of the journal is to support the research and publishing culture by ensuring that every published manuscript has an added value and thus providing international acceptance of the “readability” of the manuscripts published in the journal.