Laser Ablation Tomography for Rapid 3D Tissue Imaging and Analysis

Asheesh Lanba, B. Hall, W. Huff
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Abstract

Laser ablation tomography (LATscan) produces 3D tissue models at micron-scale resolution within a few minutes, being amenable to high-throughput applications. RGB images obtained from LATscan allow for enhanced and accurate feature segmentation and quantification. The technology uses an ultrafast, ultraviolet pulsed laser to continually ablate a sample as it is fed into the laser ablation plane. The ultrafast nature of the laser pushes the process into being athermal, minimizing structural damage to the material being imaged. The surfaces are imaged at the ablation plane. Precise motion control allows for sub-micron separation between consecutive images. The ablation results in color images due to the ultraviolet laser inducing multi-spectral fluorescence. The LATscan system can also be programmed to allow for co-registration of cross-sections under different lighting conditions. The images are then stacked, further processed and reconstructed into volume renderings with a voxel size that can go down to 0.2 µm3 for further analysis and virtual dissection. Image processing allows for the 3D visualization and quantification of desired anatomy. LATscan has been successfully applied in the fields of plant science, entomology and materials science. It shows great promise for biomedical imaging and tissue analysis, and this paper presents a few results from the LATscan imaging of murine tissue. Various murine organs have been imaged, including the gut, kidney, and brain (inside the skull). The imaging and analysis combined have the potential to provide pathologists, researchers and diagnosticians with insights and solutions not available to them before.
用于快速三维组织成像和分析的激光消融断层扫描
激光消融断层扫描(LATscan)可以在几分钟内产生微米级分辨率的3D组织模型,适用于高通量应用。从LATscan获得的RGB图像允许增强和准确的特征分割和量化。该技术使用超快的紫外脉冲激光,在将样品送入激光烧蚀平面时持续烧蚀样品。激光的超快特性使该过程成为无热的,最大限度地减少了对被成像材料的结构破坏。表面在烧蚀面成像。精确的运动控制允许在连续图像之间进行亚微米级的分离。由于紫外激光诱导多光谱荧光,烧蚀产生彩色图像。还可以对LATscan系统进行编程,以允许在不同的照明条件下对截面进行共同注册。然后将图像堆叠,进一步处理并重建为体素大小可降至0.2 μ m3的体效果图,以进行进一步分析和虚拟解剖。图像处理允许所需解剖的三维可视化和量化。LATscan在植物科学、昆虫学和材料科学等领域得到了成功的应用。它在生物医学成像和组织分析方面显示出巨大的前景,本文介绍了一些小鼠组织的LATscan成像结果。各种各样的小鼠器官已经被成像,包括肠道、肾脏和大脑(在颅骨内)。成像和分析相结合有可能为病理学家、研究人员和诊断医生提供以前无法获得的见解和解决方案。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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